Matrix metalloproteinases in human diabetic and nondiabetic vitreous
Matrix metalloproteinases in human diabetic and nondiabetic vitreous
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DOI：
10.1097/00006982-200102000-00005
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发表时间：
2001-01-01
影响因子：
3.3
通讯作者：
Tsukahara, S
中科院分区：
文献类型：
--
作者：
Jin, M;Kashiwagi, K;Tsukahara, S
目的：目的：比较糖尿病视网膜病变（diabetic retinopathy，DR）患者玻璃体中基质金属蛋白酶（matrix metalloproteinase，MMP）的活性，并探讨影响DR患者玻璃体MMP活性的因素。对在睫状体平坦部玻璃体切除术时收集的样品进行底物酶谱分析以进行MMP活性的定量分析。进行抗人MMP-1、2和9的免疫印迹以鉴定玻璃体样品中的MMP。玻璃体后脱离（PVD），玻璃体出血，增殖膜，牵引脱离，黄斑囊样水肿对MMP activity.Results的影响进行了调查：所有玻璃体样本从DR和非DR患者显示一个单一的带在72 kD的位置，对应于MMP-2。另一个99 kD的条带，对应于MMP-9，在DR样本中比非DR样本中检测到的频率显著更高：分别为45.2%和0%（P = 0.0007）。结论：MMP-9可能参与DR的发生，部分PVD与MMP-9活性有关。
Purpose: To compare matrix metalloproteinase (MMP) activities in human vitreous samples from patients with diabetic retinopathy (DR) and other vitreoretinal diseases, and to investigate the factors influencing the MMP activities in human DR vitreous samples.Methods: Thirty-one diabetic and 17 nondiabetic vitreous samples (from nine patients with macular holes and eight patients with epiretinal membranes) were examined. Samples collected at the time of pars plana vitrectomy were subjected to substrate zymography to conduct a quantitative analysis of MMP activity. Immunoblotting against antihuman MMP-1, 2, and 9 was performed to identify MMP in vitreous samples. The effects of posterior vitreous detachment (PVD), vitreous hemorrhage, proliferative membrane, traction detachment, and cystoid macular edema on MMP activities were investigated.Results: All vitreous samples from both DR and non-DR patients showed a single band at the position of 72 kD, corresponding to MMP-2. Another band at 99 kD, corresponding to MMP-9, was detected significantly more often in DR samples than in non-DR samples: 45.2% and 0%, respectively (P = 0.0007). The number of samples showing a band from MMP-9 was significantly higher in partial PVD samples than in complete PVD samples: 66.7% and 15.4%, respectively (P = 0.001).Conclusion: The results indicated that MMP-9 may be involved in DR and that partial PVD may be related to the MMP-9 activity in DR.