Hyperspectral and multispectral bioluminescence optical tomography for small animal imaging
Hyperspectral and multispectral bioluminescence optical tomography for small animal imaging
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DOI：
10.1088/0031-9155/50/23/001
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发表时间：
2005-12-07
影响因子：
3.5
通讯作者：
Leahy, RM
中科院分区：
文献类型：
--
作者：
Chaudhari, AJ;Darvas, F;Leahy, RM
对于小动物的生物发光成像研究，能够准确定位潜在生物发光源的三维（3D）分布是很重要的。由于组织光学特性的波长依赖性，从受检体发出的光源所产生的光的光谱会随着发射源的深度而变化。因此，多光谱或高光谱数据采集应该有助于深层光源的3D定位。在本文中，我们描述了一个利用光谱信息进行全3D生物发光断层图像采集和重建的框架。我们描述了正则化断层重建技术，该技术使用半无限平板或基于有限元法的光子在混浊介质中传输的扩散近似。奇异值分解分析被用于数据降维和说明使用高光谱而非消色差数据的优势。在小鼠图谱几何结构中的模拟研究表明，如果准确了解动物的光学特性，可能实现亚毫米分辨率。使用一组固定的镜子和一个单电荷耦合器件（CCD）相机同时采集动物大部分表面的多光谱成像数据。使用该系统进行的体模研究证明了我们准确定位深层点状光源的能力，并表明对于深度达6毫米的情况，可以实现1.5到2.2毫米的分辨率。我们还包括了对一只表达萤火虫荧光素酶的脑肿瘤小鼠的体内研究。重建的3D生物发光图像与磁共振图像的配准表明肿瘤具有良好的解剖定位。
For bioluminescence imaging studies in small animals, it is important to be able to accurately localize the three-dimensional (3D) distribution of the underlying bioluminescent source. The spectrum of light produced by the source that escapes the subject varies with the depth of the emission source because of the wavelength-dependence of the optical properties of tissue. Consequently, multispectral or hyperspectral data acquisition should help in the 3D localization of deep sources. In this paper, we describe a framework for fully 3D bioluminescence tomographic image acquisition and reconstruction that exploits spectral information. We describe regularized tomographic reconstruction techniques that use semi-infinite slab or FEM-based diffusion approximations of photon transport through turbid media. Singular value decomposition analysis was used for data dimensionality reduction and to illustrate the advantage of using hyperspectral rather than achromatic data. Simulation studies in an atlas-mouse geometry indicated that sub-millimeter resolution may be attainable given accurate knowledge of the optical properties of the animal. A fixed arrangement of mirrors and a single CCD camera were used for simultaneous acquisition of multispectral imaging data over most of the surface of the animal. Phantom studies conducted using this system demonstrated our ability to accurately localize deep point-like sources and show that a resolution of 1.5 to 2.2 mm for depths up to 6 mm can be achieved. We also include an in vivo study of a mouse with a brain tumour expressing firefly luciferase. Co-registration of the reconstructed 3D bioluminescent image with magnetic resonance images indicated good anatomical localization of the turnout.