Development of sensory neuropathy in streptozotocin-induced diabetic mice.
Development of sensory neuropathy in streptozotocin-induced diabetic mice.
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DOI：
10.1002/brb3.111
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发表时间：
2013-01
影响因子：
3.1
通讯作者：
Nakamura, Akihiro
中科院分区：
文献类型：
--
作者：
Murakami, Tatsufumi;Iwanaga, Takayuki;Ogawa, Yoshinao;Fujita, Yoshiaki;Sato, Eiji;Yoshitomi, Hironori;Sunada, Yoshihide;Nakamura, Akihiro
关键词：
糖尿病性多发性神经病是糖尿病的主要并发症，也是周围神经病的最常见原因。感觉支配性神经病是最常见的类型。我们以前使用链脲佐菌素（STZ）诱导的糖尿病ddY小鼠感觉神经病变，以评估血管内皮生长因子和胎盘生长因子亚型的治疗效果。在本研究中，为了表征糖尿病感觉神经病变的发展，在这些糖尿病小鼠中进行了电生理、行为和组织病理学研究。在STZ注射后1周，观察到健康小鼠和糖尿病小鼠尾神经感觉传导速度的显著差异。糖尿病小鼠在注射STZ后5周出现痛觉减退。与8周龄健康小鼠相比，17周龄健康小鼠的轴突面积和有髓纤维的髓鞘厚度增加。然而，这些增加在17周龄的糖尿病小鼠中被延迟。在无髓纤维中，与8周龄和17周龄的健康小鼠相比，17周龄的糖尿病小鼠的轴突面积显著减少。这些发现表明，在这些小鼠的糖尿病早期阶段，有髓纤维的成熟受损和无髓纤维的萎缩同时发生。该模型可用于糖尿病感觉神经病变的发病机制和治疗方法的研究。
Diabetic polyneuropathy is a major complication of diabetes and the most common cause of peripheral neuropathy. Sensory-dominant neuropathy is the most common type. We previously used streptozotocin (STZ)-induced diabetic ddY mice with sensory neuropathy to evaluate the therapeutic effects of vascular endothelial growth factor and placental growth factor isoforms. In this study, to characterize the development of diabetic sensory neuropathy, electrophysiological, behavioral, and histopathological studies were performed in these diabetic mice. A significant difference in sensory conduction velocity in the tail nerve was observed between healthy and diabetic mice at 1 week after STZ injection. Diabetic mice developed hypoalgesia at 5 weeks after STZ injection. Axon area and myelin thickness of the myelinated fibers were increased in 17-week-old healthy mice compared with those in 8-week-old healthy mice. However, these increases were retarded in 17-week-old diabetic mice. In unmyelinated fibers, axon area was significantly reduced in 17-week-old diabetic mice compared with 8- and 17-week-old healthy mice. These findings suggest that both impaired maturation of myelinated fibers and atrophy of unmyelinated fibers simultaneously occur in the early stage of diabetes in these mice. Our mouse model may be useful for studying the pathogenesis of and therapies for diabetic sensory neuropathy.
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影响因子：
7.4
作者：
Johnson MS;Ryals JM;Wright DE
通讯作者：
Wright DE
影响因子：
3.3
作者：
Christianson, J. A.;Ryals, J. M.;Wright, D. E.
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Wright, D. E.
影响因子：
3.5
作者：
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Nakamura, Akihiro
影响因子：
11.2
作者：
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通讯作者：
Losordo, Douglas
影响因子：
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作者：
Kamiya, Hideki;Zhang, Weixian;Sima, Anders A. F.
通讯作者：
Sima, Anders A. F.