A Possible Reno-protective Effect of Systemic Thermal Stimulation in a Mouse Remnant Kidney Model
A Possible Reno-protective Effect of Systemic Thermal Stimulation in a Mouse Remnant Kidney Model
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小鼠残肾模型中全身热刺激可能的肾脏保护作用
DOI：
10.11390/onki.78.118
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
Kenichiro KITAMURA
中科院分区：
文献类型：
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作者：
Yoshihiro IWASHITA;Yoshiyasu YOZA;Hiroki KAMEYAMA;Masashi MUKOYAMA;Junich IIYAMA;Kenichiro KITAMURA
目的：桑拿浴是一项受欢迎的娱乐活动，长期以来一直被用来缓解颈部僵硬和腰痛。近年来，低温桑拿已被用于治疗充血性心力衰竭（CHF）、冠状动脉疾病、慢性疲劳综合征和慢性疼痛。1960-1970年，热刺激应用于肾功能衰竭患者。我们无法找到后续报告，长期影响尚不清楚。为验证低温桑拿浴对5/6残肾小鼠的安全性及对尿蛋白排泄的影响，本实验将C57 BL/6小鼠分为4组，第1组为假手术组，第2组为非桑拿浴组组2：假手术和ST小鼠（假手术+ ST组：n = 5），组3：Nx和非ST小鼠（Nx+非ST组：n = 5），以及组4：Nx和ST小鼠（Nx+ ST组：n= 5）。结果：12周后，ST组与非ST组的肌酐清除率、体重、饮水量、尿量、血清钠、钾水平无明显差异。我们的结果显示，在Nx+ ST组中eNOS mRNA的表达显著增加相比，在Nx+ non-ST组。这些结果表明，温和的桑拿治疗可能会引起肾小球热血管舒张效应。Nx组的收缩压和尿蛋白水平在整个干预过程中没有变化。结论：没有明显的不良事件与低温桑拿。因此，该研究环境在CKD模型小鼠中是安全的。低温桑拿组大鼠肾脏eNOS mRNA表达增加。本研究结果表明，ST可能通过刺激CKD模型小鼠肾脏NO的产生来抑制肾小球高血压，从而提供肾脏保护作用。
Objective: Sauna bathing is a popular recreational activity and has long since been used to relieve stiff necks and low back pain. Recently, low-temperature sauna has been used to treat congestive heart failure (CHF), coronary artery diseases, chronic fatigue syndrome, and chronic pain. During 1960-1970, thermal stimulation was applied to the patients with renal failure. We could not find the subsequent reports, and the long-term effects are unclear. The purpose of this experiment was to verify the safety of systemic low-temperature sauna treatment (ST) for the 5/6 remnant kidney mouse and to examine the effect of ST on urinary protein excretion.Materials and Methods: The C57BL/6 mice were divided into the following 4 groups; group 1: sham-operated and non-sauna treatment mice (sham+ non-ST group: n= 5), group 2: sham-operated and ST mice (sham+ ST group: n= 5), group 3: Nx and non-ST mice (Nx+ non-ST group: n= 5), and group 4: Nx and ST mice (Nx+ ST group: n= 5). Mice received ST at 41 Cfor 15 min and at 32 Cfor 20 min for 12 weeks using a natural convection dry sauna system.Results: After 12 weeks of ST, no differences were observed in creatinine clearance, body weight, fluid intake, urine volume, serum sodium and potassium levels between ST and non-ST groups. Our results showed a significant increase in eNOS mRNA expression in the Nx+ ST group compared to that in the Nx+ non-ST group. These results suggest the possibility that mild sauna treatment induces thermal vasodilation effects on glomerulus. Systolic blood pressure and urine protein levels in the Nx groups did not change throughout the intervention.Conclusion: There are no clear adverse events associated with low-temperature sauna. Therefore, this study setting is safe in the CKD model mouse. Renal eNOS mRNA expression was increased by the low-temperature sauna. The present results suggest the possibility that ST might provide a renal protective effect by suppressing glomerular hypertension via stimulation of renal NO production in the CKD model mouse.