Pathogenomic comparison of human extraintestinal and avian pathogenic Escherichia coli - Search for factors involved in host specificity or zoonotic potential
Pathogenomic comparison of human extraintestinal and avian pathogenic Escherichia coli - Search for factors involved in host specificity or zoonotic potential
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DOI：
10.1016/j.micpath.2010.05.004
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发表时间：
2010-09-01
影响因子：
3.8
通讯作者：
Dobrindt, Ulrich
中科院分区：
文献类型：
--
作者：
Bauchart, Philippe;Germon, Pierre;Dobrindt, Ulrich
禽致病性大肠杆菌（APEC）和人肠外致病性大肠杆菌（E. coli）。大肠杆菌（ExPEC）在人类和动物中引起各种疾病，并且不能通过分子流行病学和基因组含量清楚地区分。我们表征了8个代表性的人类ExPEC和APEC变异体的特征，以支持人畜共患病的可能性或指示参与宿主特异性的因素。这些菌株在致病性、毒力基因含量和粘附素等位变异方面非常相似。未发现1型、P型、S/F1 C菌毛、卷曲、鞭毛、大肠杆菌素和需氧菌素表达或体内毒力的宿主或血清群特异性差异。我们对响应人（37 ℃）或禽（41 ℃）体温的人ExPEC IHE 3034和APEC BEN 374进行转录组分析。两种分离株在两种温度下均显示出相似的转录谱。运动性/趋化性基因的转录水平在41 ℃时受到抑制。参与酸胁迫抗性的hdeAB和cadA基因，虽然经常在41 ℃诱导，但不能与宿主特异性相关。除了菌株特异性效应外，两种菌株在人类或鸟类体温下的共同行为支持了某些人类ExPEC和APEC变种存在潜在人畜共患风险的观点。(C)2010爱思唯尔有限公司保留所有权利。
Avian pathogenic Escherichia coli (APEC) and human extraintestinal pathogenic E. coli (ExPEC) cause various diseases in humans and animals and cannot be clearly distinguished by molecular epidemiology and genome content. We characterized traits of eight representative human ExPEC and APEC variants to either support the zoonotic potential or indicate factors involved in host specificity. These strains were very similar regarding phylogeny, virulence gene content and allelic variation of adhesins. Host- or serogroup-specific differences in type 1-, P-, S/F1C-fimbriae, curli, flagella, colicin and aerobactin expression or in vivo virulence were not found. Serogroup-dependent differences in genome content may depend on the phylogenetic background.To identify traits involved in host specificity, we performed transcriptome analysis of human ExPEC IHE3034 and APEC BEN374 in response to human (37 degrees C) or avian (41 degrees C) body temperature. Both isolates displayed similar transcriptional profiles at both temperatures. Transcript levels of motility/chemotaxis genes were repressed at 41 degrees C. The hdeAB and cadA genes involved in acid stress resistance, although often induced at 41 degrees C, could not be correlated with host specificity. Beside strain-specific effects, the common behavior of both strains at human or avian body temperature supports the idea of a potential zoonotic risk of certain human ExPEC and APEC variants. (C) 2010 Elsevier Ltd. All rights reserved.