Alteration of CD4CD25Foxp3 T cell level in Kawasaki disease.
Alteration of CD4CD25Foxp3 T cell level in Kawasaki disease.
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川崎病中CD4CD25FOXP3 T细胞水平的改变。
DOI：
10.3345/kjp.2011.54.4.157
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发表时间：
2011-04
影响因子：
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通讯作者：
Lee KC
中科院分区：
文献类型：
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作者：
Sohn SY;Song YW;Yeo YK;Kim YK;Jang GY;Woo CW;Lee JH;Lee KC
在川崎急性期，过度的促炎反应提示免疫失调在川崎病发病机制中的作用。我们研究了调节性T细胞的分布及其与KD临床病程的相关性。收集17例KD患儿急性发热期和亚急性无热期外周血单个核细胞。采用流式细胞术分析表达CD 4、CD 25和Foxp 3的T细胞，并将结果与KD的临床病程相关。亚急性无热期外周血CD 4 + CD 25 highFoxp 3 + T细胞占CD 4 + T细胞的比例显著高于急性发热期（1.10%± 1.22%vs.0.55% ± 0.53%，P=0.049）。虽然CD 4 + CD 25 lowFoxp 3 + T细胞和CD 4 + CD 25-Foxp 3 + T细胞水平仅轻微改变，但亚急性无发热期CD 4 + CD 25 + Foxp 3- T细胞在CD 4 + T细胞中的百分比显著低于急性发热期（2.96%±1.95% vs. 5.64%± 5.69%，P=0.036）。因此，亚急性无发热期的CD 25 highFoxp 3 + T细胞与CD 25 + Foxp 3- T细胞的比例高于急性发热期（0.45%±0.57% vs. 0.13%± 0.13%，P=0.038）。CD 4 + CD 25 highFoxp 3 + T细胞减少和/或CD 4 + CD 25 highFoxp 3 + T细胞与CD 4 + CD 25 + Foxp 3- T细胞比例失衡可能在KD的发生中起作用。考虑到所有KD患者均接受静脉注射免疫球蛋白（IVIG）治疗，在亚急性发热期恢复CD 4 + CD 25 highFoxp 3 + T细胞可能是IVIG的一种机制。
Exaggerated pro-inflammatory reactions during the acute phase of Kawasaki disease (KD) suggest the role of immune dysregulation in the pathogenesis of KD. We investigated the profiles of T regulatory cells and their correlation with the clinical course of KD. Peripheral blood mononuclear cells were collected from 17 KD patients during acute febrile and subacute afebrile phases. T cells expressing CD4, CD25, and Foxp3 were analyzed using flow cytometry, and the results were correlated with the clinical course of KD. The percentage of circulating CD4+CD25highFoxp3+ T cells among CD4+ T cells was significantly higher during the subacute afebrile phase than during the acute febrile phase (1.10%±1.22% vs. 0.55%±0.53%, P=0.049). Although levels of CD4+CD25lowFoxp3+ T cells and CD4+CD25-Foxp3+ T cells were only slightly altered, the percentage of CD4+CD25+Foxp3- T cells among CD4+ T cells was significantly lower during the subacute afebrile phase than during the acute febrile phase (2.96%±1.95% vs. 5.64%±5.69%, P=0.036). Consequently, the ratio of CD25highFoxp3+ T cells to CD25+Foxp3- T cells was higher during the subacute afebrile phase than during the acute febrile phase (0.45%±0.57% vs. 0.13%±0.13%, P=0.038). Decreased CD4+CD25highFoxp3+ T cells and/or an imbalanced ratio of CD4+CD25highFoxp3+ T cells to CD4+CD25+Foxp3- T cells might play a role in KD development. Considering that all KD patients were treated with intravenous immunoglobulin (IVIG), recovery of CD4+CD25highFoxp3+ T cells during the subacute afebrile phase could be a mechanism of IVIG.