Application of Fluolid-Orange-labeled probes for DNA microarray and immunological assays
Application of Fluolid-Orange-labeled probes for DNA microarray and immunological assays
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DOI：
10.1007/s10529-011-0646-0
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发表时间：
2011-09-01
影响因子：
2.7
通讯作者：
Kiyama, Ryoiti
中科院分区：
文献类型：
--
作者：
Zhu, Yun;Ogaeri, Takunori;Kiyama, Ryoiti
Fluolid-Orange是一种新型的荧光染料，用于DNA微阵列和免疫分析。Fluolid-Orange标记的探针（DNA和IgG）通过激光光漂白以及在热和干燥条件下进行检查是稳定的。进行了统计分析，以评估微阵列检测的再现性，而阶段特异性的标记蛋白，Kank 1和calretinin的免疫染色，肾癌进行，都给出了令人满意的结果。染料的稳定性应提供优势，用于储存荧光标记的探针和重新检查标本后，在遗传和病理诊断。
The usefulness of Fluolid-Orange, a novel fluorescent dye, for DNA microarray and immunological assays has been examined. Fluolid-Orange-labeled probes (DNA and IgG) were stable as examined by laser-photo-bleaching and under heat and dry conditions. Statistical analyses were performed to evaluate the reproducibility of the microarray assay, while stage-specific immunostaining of marker proteins, Kank1 and calretinin, was performed for renal cancers, both giving satisfactory results. The stability of the dye should provide advantages for storing fluorescently labeled probes and re-examining the specimens later in genetic and pathological diagnostics.