Reduction in lateral lipid mobility of lipid bilayer membrane by atmospheric pressure plasma irradiation
Reduction in lateral lipid mobility of lipid bilayer membrane by atmospheric pressure plasma irradiation
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DOI：
10.7567/jjap.55.03df05
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发表时间：
2016-02
影响因子：
1.5
通讯作者：
Y. Suda;R. Tero;Ryuma Yamashita;Kota Yusa;H. Takikawa
中科院分区：
文献类型：
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作者：
Y. Suda;R. Tero;Ryuma Yamashita;Kota Yusa;H. Takikawa
等离子体医学是一个新兴的研究领域，其中研究了放电的各种应用，特别是在大气压下以非平衡等离子体的形式，例如，将等离子体应用于生物靶标以实现各种目的，例如选择性杀死肿瘤细胞和止血。我们重点研究人工细胞膜系统在固液界面的行为。为了评估横向脂质迁移率，我们通过共焦激光扫描显微镜测量了由二油酰磷脂酰胆碱组成的支撑脂质双层（SLB）的扩散系数，并在光漂白后恢复荧光。发现扩散系数因等离子体照射而降低，并且扩散系数降低速率随着等离子体功率的增加而增加。我们研究了平衡化学物质 H2O2 的刺激对 SLB 的影响，并证实扩散系数至少在 H2O2 浓度达到 5 mM 时没有变化。这些结果表明，等离子体产生的瞬态活性物质在 SLB 流动性降低中发挥着关键作用。讨论了两种生成的主要氧化脂质种类，羟基或过氧化磷脂酰胆碱 (PC) 和以醛基或羧基封端的酰基链截短的 PC，对横向脂质迁移率的影响。
Plasma medicine is an emerging research field in which various applications of electrical discharge, especially in the form of nonequilibrium plasma at atmospheric pressure, are examined, for example, the application of plasma to biological targets for various purposes such as selective killing of tumor cells and blood stanching. We have focused on the behavior of an artificial cell membrane system at the solid–liquid interface. To evaluate the lateral lipid mobility, we measured the diffusion coefficient of the supported lipid bilayer (SLB) composed of dioleoylphosphatidylcholine with fluorescence recovery after photobleaching by confocal laser scanning microscopy. It was found that the diffusion coefficient was decreased by plasma irradiation and that the diffusion coefficient decreasing rate proceeded with increasing plasma power. We investigated the effects of stimulation with an equilibrium chemical, H2O2, on the SLB and confirmed that the diffusion coefficient did not change at least up to a H2O2 concentration of 5 mM. These results indicate that transient active species generated by plasma play critical roles in the reduction in SLB fluidity. The effects of the two generated major oxidized lipid species, hydroxyl- or hydroperoxy-phosphatidylcholine (PC) and acyl-chain-truncated PCs terminated with aldehyde or carboxyl group, on lateral lipid mobility are discussed.