SOMATIC MUTATION OF THE APC GENE IN GASTRIC-CANCER - FREQUENT MUTATIONS IN VERY WELL DIFFERENTIATED ADENOCARCINOMA AND SIGNET-RING CELL-CARCINOMA
SOMATIC MUTATION OF THE APC GENE IN GASTRIC-CANCER - FREQUENT MUTATIONS IN VERY WELL DIFFERENTIATED ADENOCARCINOMA AND SIGNET-RING CELL-CARCINOMA
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DOI：
10.1093/hmg/1.8.559
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发表时间：
1992-11-01
影响因子：
3.5
通讯作者：
HORII, A
中科院分区：
文献类型：
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作者：
NAKATSURU, S;YANAGISAWA, A;HORII, A
我们寻找体细胞突变的腺瘤性息肉病大肠杆菌（APC）基因的DNA样本分离自57散发性胃癌，通过核糖核酸酶（RNase）的保护分析加上DNA扩增的聚合酶链反应（PCR）。检查30%的APC编码区，包括已知结直肠肿瘤中体细胞突变聚集的区域，我们在12个肿瘤中检测到体细胞突变; 17个非常高分化腺癌中有7个，19个高分化或中等分化腺癌中有2个，10个印戒细胞癌中有3个。到目前为止，在11例低分化腺癌中未发现体细胞突变。在12个肿瘤中发现的17个体细胞突变中，有8个由于无义突变和1-，2-或5-bp缺失而导致基因产物截短;其他9个是改变氨基酸的点突变。我们的研究结果表明，APC的失活在某些胃癌，特别是分化良好的腺癌和印戒细胞癌的发展中发挥作用。
We searched for somatic mutations of the adenomatous polyposis coli (APC) gene in DNA samples isolated from 57 sporadic gastric cancers, by means of a ribonuclease (RNase) protection analysis coupled with DNA amplification by the polymerase chain reaction (PCR). Examining 30% of the APC coding region, including a region where somatic mutations in colorectal tumors are known to be clustered, we detected somatic mutations in 12 tumors; seven in 17 very well differentiated adenocarcinomas, two in 19 well or moderately differentiated adenocarcinomas, and three in ten signet-ring cell carcinomas. So far, no somatic mutations have been identified in 11 poorly differentiated adenocarcinomas. Eight of the 17 somatic mutations found in 12 tumors caused truncation of the gene product due to a nonsense mutation and a 1-, 2- or 5-bp deletion; nine others were point mutations that altered amino acids. Our results suggest that inactivation of APC plays a role in development of some gastric cancers, particularly very well differentiated adenocarcinomas and signet-ring cell carcinomas.