Ligand Engineering via Yeast Surface Display and Adherent Cell Panning.
Ligand Engineering via Yeast Surface Display and Adherent Cell Panning.
复制标题
通过酵母表面展示和贴壁细胞淘选进行配体工程。
DOI：
10.1007/978-1-4939-9853-1_17
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
Hackel,BenjaminJ
中科院分区：
文献类型：
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作者：
Stern,LawrenceA;Lown,PatrickS;Hackel,BenjaminJ
高通量配体的发现和进化-通过基因型-表型连锁策略-赋予分子靶向治疗，诊断和基础科学。在这些选择中保持高质量的靶抗原，特别是对于膜靶，通常是一个技术挑战。在表达分子靶标的完整哺乳动物细胞上淘选酵母展示的配体文库已成为一种有效的策略。在本文中，我们描述了用于通过这种方法选择靶结合配体的技术，包括使用靶阴性细胞来耗尽非特异性结合剂和亲合力降低以优先选择高亲和力配体。
High-throughput ligand discovery and evolution—via genotype-phenotype linkage strategies—empower molecularly targeted therapy, diagnostics, and fundamental science. Maintaining high-quality target antigen in these selections, particularly for membrane targets, is often a technical challenge. Panning yeast-displayed ligand libraries on intact mammalian cells expressing the molecular target has emerged as an effective strategy. Herein we describe the techniques used to select target-binding ligands via this approach including the use of target-negative cells to deplete non-specific binders and avidity reduction to preferentially select high-affinity ligands.