Antifungal activities of polyhexamethylene biguanide and polyhexamethylene guanide against the citrus sour rot pathogen Geotrichum citri-aurantii in vitro and in vivo
Antifungal activities of polyhexamethylene biguanide and polyhexamethylene guanide against the citrus sour rot pathogen Geotrichum citri-aurantii in vitro and in vivo
复制标题
聚六亚甲基双胍和聚六亚甲基胍对柑橘酸腐病菌柑橘地霉的体外和体内抗真菌活性
DOI：
10.1016/j.postharvbio.2011.03.002
复制
发表时间：
2011-08-01
影响因子：
7
通讯作者：
Duan, Xuewu
中科院分区：
文献类型：
--
作者：
Feng, Linyan;Wu, Fuwang;Duan, Xuewu
聚六亚甲基双胍（PHMB）和聚六亚甲基胍（PHMG）是广谱抗菌剂。进行实验以研究 PHMB 和 PHMG 对主要柑橘酸腐病菌柑橘地霉 (Geotrichum citri-aurantii) 的体外和体内抗真菌活性。 PHMG 和 PHMB 处理显着抑制体外柑橘 G. citri-aurantii 的节分孢子萌发和菌丝生长。 5 mg/L 的 PHMG 和 PHMB 分别抑制 97.8% 和 95.8% 的柑橘小分生孢子萌发。光学显微镜、扫描电子显微镜（SEM）和透射电子显微镜（TEM）的观察进一步显示了PHMG或PHMB处理引起的超微结构改变，包括菌丝扭曲、变形和凹陷塌陷，菌丝和节分孢子的质膜破坏，以及节分孢子的细胞壁损伤。 PHMG 和 PHMB 的使用显着（P
Polyhexamethylene biguanide (PHMB) and polyhexamethylene guanide (PHMG) are broad-spectrum antibacterial agents. Experiments were conducted to investigate the antifungal activities of PHMB and PHMG against a major citrus sour rot pathogen Geotrichum citri-aurantii both in vitro and in vivo. PHMG and PHMB treatments significantly inhibited arthroconidia germination and mycelial growth of G. citri-aurantii in vitro. PHMG and PHMB at 5 mg/L inhibited 97.8% and 95.8% of the germination of G. citri-aurantii arthroconidia, respectively. Observations by light microscopy, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) further showed ultrastructural alterations caused by PHMG or PHMB treatments, including distortion, deformation and concave collapse in hyphae, plasma membrane disruption in both hyphae and arthroconidia, and cell wall damage in arthroconidia. Use of PHMG and PHMB significantly (P