Fibrosis biomarkers in workers exposed to MWCNTs.
Fibrosis biomarkers in workers exposed to MWCNTs.
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DOI：
10.1016/j.taap.2016.02.016
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发表时间：
2016-05-15
影响因子：
3.8
通讯作者：
Shvedova AA
中科院分区：
文献类型：
--
作者：
Fatkhutdinova LM;Khaliullin TO;Vasil'yeva OL;Zalyalov RR;Mustafin IG;Kisin ER;Birch ME;Yanamala N;Shvedova AA
多壁碳纳米管(MWCNT)以其独特的物理化学性质提供了许多技术优势，预计将推动下一代制造业的增长。由于多壁碳纳米管已经在建筑、工程、能源生产、空间探索和生物医学等不同行业中得到了应用，大量的多壁碳纳米管可能会接触到环境，并在不经意间导致人类暴露。这就需要紧急评估它们对人类的潜在健康影响。目前的研究是在NanotechCenter Ltd.Enterprise(俄罗斯坦博夫)进行的，在那里报告了MWCNT的大规模制造以及相对较高的职业暴露水平。这项小型横断面研究的目的是评估职业性接触多壁碳纳米管的潜在生物标志物。所有空气样本都是在工作场所从特定区域和个人呼吸区收集的，使用基于过滤器的设备来定量元素碳，并通过透射电子显微镜进行颗粒物分析。分别从接触和非接触MWCNT工人的鼻腔灌洗、诱导痰和血清中获取生物液，以评估炎症和纤维化标志物。结果发现，接触多壁碳纳米管后，痰标本中IL-1β、IL-6、肿瘤坏死因子-α、炎性细胞因子和肺间质疾病的血清学标志物KL-6水平均显著升高。此外，年轻接触者血清中转化生长因子-β-1水平升高。总体而言，这项研究的结果揭示了制造多壁碳纳米管的工人的生物体液中炎症和纤维化生物标志物的积累。因此，在横断面流行病学研究中，应考虑所分析的生物标志物来评估职业性接触MWCNT的健康影响。
Multi-walled carbon nanotubes (MWCNT) with their unique physico-chemical properties offer numerous technological advantages and are projected to drive the next generation of manufacturing growth. As MWCNT have already found utility in different industries including construction, engineering, energy production, space exploration and biomedicine, large quantities of MWCNT may reach the environment and inadvertently lead to human exposure. This necessitates the urgent assessment of their potential health effects in humans. The current study was carried out at NanotechCenter Ltd. Enterprise (Tambov, Russia) where large-scale manufacturing of MWCNT along with relatively high occupational exposure levels was reported. The goal of this small cross-sectional study was to evaluate potential biomarkers during occupational exposure to MWCNT. All air samples were collected at the workplaces from both specific areas and personal breathing zones using filter-based devices to quantitate elemental carbon and perform particle analysis by TEM. Biological fluids of nasal lavage, induced sputum and blood serum were obtained from MWCNT-exposed and non-exposed workers for assessment of inflammatory and fibrotic markers. It was found that exposure to MWCNTs caused significant increase in IL-1β, IL6, TNF-α, inflammatory cytokines and KL-6, a serological biomarker for interstitial lung disease in collected sputum samples. Moreover, the level of TGF-β1 was increased in serum obtained from young exposed workers. Overall, the results from this study revealed accumulation of inflammatory and fibrotic biomarkers in biofluids of workers manufacturing MWCNTs. Therefore, the biomarkers analyzed should be considered for the assessment of health effects of occupational exposure to MWCNT in cross-sectional epidemiological studies.