Differential mRNA and Long Noncoding RNA Expression Profiles in Umbilical Cord Blood Exosomes from Gestational Diabetes Mellitus Patients
Differential mRNA and Long Noncoding RNA Expression Profiles in Umbilical Cord Blood Exosomes from Gestational Diabetes Mellitus Patients
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DOI：
10.1089/dna.2020.5783
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发表时间：
2020-09-28
影响因子：
3.1
通讯作者：
Wen, Juan
中科院分区：
文献类型：
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作者：
Cao, Minkai;Zhang, Le;Wen, Juan
背景和目的：外泌体含有大量RNA，并在细胞或器官之间转移它们，从而建立细胞间或器官间通讯。脐带血外泌体中的 mRNA 和长非编码 RNA (lncRNA) 在妊娠期糖尿病 (GDM) 发生和胎儿生长中的作用仍知之甚少。我们的目的是建立 GDM 患者脐带血外泌体与正常对照相比的差异 mRNA 和 lncRNA 表达谱。结果：利用微阵列技术，我们鉴定出 GDM 患者脐带血外泌体中与对照组相比存在差异表达的 84 个 mRNA 和 256 个 lncRNA。蛋白质-蛋白质相互作用网络表明差异表达的mRNA与胰高血糖素信号通路有关，胰高血糖素信号通路是GDM相关的重要通路。对与差异表达的 lncRNA 相关的 mRNA 进行基因本体论和京都基因和基因组百科全书 (KEGG) 生物途径分析。结果表明，代谢过程、生长和发育显着丰富，这对 GDM 发育和胎儿生长至关重要。此外，构建通路网络以揭示GDM的关键通路，如代谢通路和胰岛素信号通路。进一步的lncRNA/miRNA相互作用分析表明，大多数外泌体lncRNA都含有miRNA结合位点，其中一些与GDM相关。结论：这些结果表明，外泌体mRNA和lncRNA在GDM患者的脐带血中异常表达，并在GDM的发生和胎儿生长中发挥潜在作用。
Background and Aims:Exosomes contain numerous RNAs and transfer them between cells or organs, thereby establishing intercellular or interorgan communication. The roles of mRNAs and long noncoding RNAs (lncRNAs) from umbilical cord blood exosomes in gestational diabetes mellitus (GDM) occurrence and fetus growth remain poorly understood. We aimed to establish the differential mRNA and lncRNA expression profiles in umbilical cord blood exosomes from GDM patients compared with normal controls. Results:Using microarray technology, we identified 84 mRNAs and 256 lncRNAs as differentially expressed in umbilical cord blood exosomes of GDM patients compared with controls. The protein-protein interaction network revealed that the differentially expressed mRNAs were associated with glucagon signaling pathway, an important GDM-related pathway. Gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) biological pathway analyses were performed for mRNAs associated with differentially expressed lncRNAs. The results indicated that metabolic process, growth, and development were significantly enriched, which are important in GDM development and fetus growth. Moreover, pathway network was constructed to reveal the key pathways in GDM, such as metabolic pathways and insulin signaling pathway. Further lncRNA/miRNA interaction analysis showed that most of the exosomal lncRNAs harbored miRNA binding sites, and some were associated with GDM. Conclusion:These results showed that exosomal mRNAs and lncRNAs are aberrantly expressed in the umbilical cord blood of GDM patients and play potential roles in GDM development and fetus growth.