A newly identified photolyase from Arthrospira platensis possesses a unique methenyltetrahydrofolate (MTHF) chromophore‐binding pattern
A newly identified photolyase from Arthrospira platensis possesses a unique methenyltetrahydrofolate (MTHF) chromophore‐binding pattern
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一种新鉴定的来自节旋藻的光裂合酶具有独特的次甲基四氢叶酸发色团结合模式。
DOI：
10.1002/1873-3468.13657
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发表时间：
2019
期刊：
影响因子：
3.5
通讯作者：
Xu Li
中科院分区：
文献类型：
--
作者：
Hui Yan;Kongfu Zhu;Maikun Teng;Xu Li
环丁烷嘧啶二聚体（CPD）作为一种常见的紫外线辐射引起的DNA损伤，常导致皮肤癌。在这里，我们从钝顶螺旋藻中鉴定出一种光解酶（命名为Ap ‐phr），几个世纪以来一直被认为是对人类安全的生物体，它可以在体外有效地修复ssDNA和dsDNA中的CPD损伤。Ap ‐phr的1.6 nm分辨率晶体结构显示，它具有独特的亚甲基四氢叶酸生色团结合模式，具有高能量转移效率。我们对Ap ‐phr的研究突出了其在化妆品，工业和美容医学应用中的潜在用途。
Cyclobutane pyrimidine dimers (CPD), as a common DNA damage caused by UV radiation, often lead to skin cancer. Here, we identified a photolyase from the algaArthrospira platensis(designated asAp‐phr), which has been regarded as a safe organism for humans for centuries, that can efficiently repair CPD lesions in ssDNA and dsDNAin vitro. The 1.6 Å resolution crystal structure ofAp‐phr revealed that it possesses a unique methenyltetrahydrofolate chromophore‐binding pattern with high energy transfer efficiency. Our study ofAp‐phr highlights its potential use in cosmetic, industrial and aesthetic medicine applications.