PAX5 haploinsufficiency induce cancer cell dormancy in Raji cells
PAX5 haploinsufficiency induce cancer cell dormancy in Raji cells
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PAX5单倍体不足诱导Raji细胞中的癌细胞休眠
DOI：
10.1016/j.yexcr.2018.02.010
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发表时间：
2018-06-01
影响因子：
3.7
通讯作者：
Zhou, Jianfeng
中科院分区：
文献类型：
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作者：
Liang, Xue;Gu, Jia;Zhou, Jianfeng
PAX 5突变在白血病的发生和复发中起重要作用，癌细胞休眠参与了肿瘤的复发，但关于PAX 5突变诱导癌细胞休眠的研究尚未见报道。利用基因编辑技术构建PAX 5缺失Raji细胞系，评价细胞系的休眠生物学特性。结果表明PAX 5单倍不足抑制Raji细胞的增殖，诱导Raji细胞发生G 0/G1期阻滞，降低化疗敏感性。PAX 5突变的Raji细胞成瘤率降低。我们的结果表明PAX 5不足诱导Raji细胞中的癌细胞休眠。
PAX5 mutations have important role in leukemogenesis and leukemia relapse, cancer cell dormancy participates in cancer relapse, but there was no report about PAX5 mutation inducing cancer cell dormancy. we constructed the PAX5 deletion Raji cell lines using gene editing technology, evaluated dormancy biological characteristics of cell lines. Our results showed PAX5 haploinsufficiency restrained the proliferation of Raji cells, induced G0/G1 arrest of Raji cells, reduced chemotherapy sensitivity. The tumor formation rate reduced in PAX5 mutation Raji cells. Our results showed PAX5 insufficiency induced cancer cell dormancy in Raji cell.