Activation of cloned human CD4+ Th1 and Th2 cells by blood dendritic cells.
Activation of cloned human CD4+ Th1 and Th2 cells by blood dendritic cells.
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血液树突状细胞激活克隆的人类 CD4 Th1 和 Th2 细胞。
DOI：
10.1046/j.1365-3083.1996.d01-270.x
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发表时间：
1996
影响因子：
3.7
通讯作者：
Spiegelberg,HL
中科院分区：
文献类型：
--
作者：
Roth,R;Spiegelberg,HL
树突状细胞（DC）是激活初始T细胞的最有效的抗原呈递细胞（APC），在混合淋巴细胞反应中比单核细胞（Mφ）的APC效力高10-100倍。本研究比较了人血DC、Mφ和B细胞对克隆的黑麦草变应原Lol pI特异性的CD 4 + Th 1和Th 2细胞的激活能力。在Lol pI存在的情况下，所有三种类型的APC以相似的程度激活Th 1和Th 2细胞，如T细胞增殖和干扰素γ、白细胞介素2（IL-2）或IL-4分泌所示。 然而，在低APC：T细胞比例下，Mφ是对Th 1和Th 2细胞最有效的APC，其次是DC和B细胞。 使用针对HLA-DR高-DC、CD 14 pos-Mφ和CD 19 pos-B细胞的荧光细胞分选，通过阴性选择分离或通过阳性选择高度纯化的APC制备物观察到该等级。数据表明，与已报道的幼稚T细胞相反，人血DC激活克隆的记忆性Th 1和Th 2细胞的程度与Mφ和B细胞相似，这可能是因为激活记忆型T细胞的要求不如幼稚T细胞严格。
Dendritic cells (DC) have been reported to be the most potent antigen‐presenting cells (APC) for the activation of naive T cells and to be 10–100‐fold more potent APC than monocytes (Mφ) in the mixed lymphocyte reaction. In this study the authors compared human blood DC with Mφ and B cells for their ability to activate cloned rye grass allergenLol pI specific CD4+Th1and Th2cells. In the presence ofLol pI, all three types of APC activated Th1and Th2cells to a similar extent, as shown by T‐cell proliferation and interferon‐γ, interleukin‐2 (IL‐2) or IL‐4 secretion. However, at low APC : T cell ratios, Mφ were the most potent APC for both Th1and Th2cells followed in decreasing order by DC and B cells. This hierarchy was observed with APC preparations isolated by negative selection or highly purified by positive selection using fluorescent cell sorting for HLA‐DRhigh‐DC, CD14pos‐Mφ and CD19pos‐B cells. The data demonstrate that, in contrast to what has been reported for naive T cells, human blood DC activate cloned memory Th1and Th2cells to a similar extent as Mφ and B cells presumably because the requirements for activation of memory type T cells are less stringent than those for naive T cells.