Influence of cytoplasmic deletions on the filopodia-inducing effect of syndecan-3
Influence of cytoplasmic deletions on the filopodia-inducing effect of syndecan-3
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DOI：
10.1016/j.cellbi.2004.08.003
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发表时间：
2004-01-01
影响因子：
3.9
通讯作者：
Reina, M
中科院分区：
文献类型：
--
作者：
Berndt, C;Montañez, E;Reina, M
Syndecans、跨膜型硫酸乙酰肝素蛋白多糖(HSPG)通过调节细胞与细胞、细胞与基质的黏附，控制细胞的运动和形状。Syndecan细胞质结构域非常短(约30个氨基酸)，分为两个恒定区(C1和C2)，由一个可变区(V)隔开。在这里，我们试图定位负责Syndecan-3丝状伪足诱导作用的细胞质区域。我们发现，只有C1-区是这种效应所必需的。此外。C2区的缺失导致了广泛的膜气泡。然而，整个细胞质区域的消除并不影响Syndecan-3向质膜的输送。这些结果表明，Syndecan-3胞质结构域的不同区域可能通过与不同的蛋白质结合而具有不同的功能。(C)2004年国际细胞生物学联合会。爱思唯尔有限公司出版。保留所有权利。
Syndecans, transmembrane heparan sulfate proteoglycans (HSPG), mediate cell-cell and cell-matrix adhesion thereby, controlling cell movement and shape. Syndecan cytoplasmic domains are very short (ca. 30 amino acids) and divided into two constant regions (C1 and C2) separated by one variable (V) region. Here we attempted to map the cytoplasmic region responsible for the filopodia-inducing effect of syndecan-3. We found that only the C1-region was necessary for this effect. In addition. the deletion of the C2-region led to extensive membrane blebbing. Nevertheless, the elimination of the entire cytoplasmic region did not affect delivery of syndecan-3 to the plasma membrane. These results indicate that the different regions of syndecan-3 cytoplasmic domain have different functions probably by binding to distinct proteins. (C) 2004 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.