Bioprospecting for antagonistic Penicillium strains as a resource of new antitumor compounds
Bioprospecting for antagonistic Penicillium strains as a resource of new antitumor compounds
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DOI：
10.1007/s11274-007-9455-y
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发表时间：
2008-02-01
影响因子：
4.1
通讯作者：
Tufano, Maria Antonietta
中科院分区：
文献类型：
--
作者：
Nicoletti, Rosario;Buommino, Elisabetta;Tufano, Maria Antonietta
微生物正越来越多地被用作新药物的来源。土壤真菌在这方面特别有希望，因为它们的生物群落竞争力往往建立在抗生素和其他抑制物质的生产基础上。大约15株具有一定抗真菌能力的青霉菌株被评估为可能的抗肿瘤产品来源，基于它们的培养提取物对人肿瘤细胞株的抗增殖和促凋亡作用。大约有12个菌株表现出一定程度的抗肿瘤特性，在一些情况下，这些特性与从培养物提取物中提纯的外分泌物本身的效果相对应。这项研究表明，可以通过直接在肿瘤细胞上分析真菌提取物来快速筛选真菌分离物，其好处是将鉴定生物活性化合物所需的工作限制在较少数量的有希望的菌株上。
Microorganisms are increasingly exploited as a source of new pharmaceuticals. Soil fungi are particularly promising to this regard since their biocenotic competitiveness is often based on the production of antibiotics and other inhibitory substances. About 15 Penicillium strains possessing some extent of antifungal ability were evaluated as a possible source of antitumor products based on the antiproliferative and pro-apoptotic effects of their culture extracts on human tumor cell lines. About 12 strains showed some extent of antitumor properties that in several cases corresponded to the effect of the extrolites purified from the culture extracts themselves. The study showed that a quick screening of fungal isolates can be operated by assaying their fungal extracts on tumor cells directly, with the ensuing advantage of restricting the work required for the characterization of the bioactive compounds to a reduced number of promising strains.