Enhanced hydrogen production by a newly described heterotrophic marine bacterium, Vibrio tritonius strain AM2, using seaweed as the feedstock
Enhanced hydrogen production by a newly described heterotrophic marine bacterium, Vibrio tritonius strain AM2, using seaweed as the feedstock
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DOI：
10.1016/j.ijhydene.2014.02.164
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发表时间：
2014-05
影响因子：
7.2
通讯作者：
Yuta Matsumura;Kazumichi Sato;N. Al‐saari;S. Nakagawa;T. Sawabe
中科院分区：
文献类型：
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作者：
Yuta Matsumura;Kazumichi Sato;N. Al‐saari;S. Nakagawa;T. Sawabe
为了更稳定地利用非食用原料生产生物氢(BioH2)，稳定的海洋生物原料制备和高效的海洋细菌盐渍条件下生物制氢系统是需要开发的两项重要关键技术。从海洋无脊椎动物肠道中分离出的三特弧菌AM2在添加海藻碳水化合物甘露醇的不同条件下(初始浓度为2.25%(w/v))进行了培养，以评价其产氢性能。在pH=6和37℃时，生物氢的最大摩尔产率为1.7摩尔/摩尔甘露醇。在pH 5.5~7.5范围内，甘露醇培养细胞的生物氢产量高于葡萄糖培养细胞。与葡萄糖相比，甘露醇可能是AM2菌株产H_2的更好的底物。发酵产物分析表明，菌株AM2可能利用甲酸盐-氢气途径生产生物H_2。此外，菌株AM2能够从含有31.1%干重甘露醇的褐藻粉中产生氢气。当海藻原料中含有氢气/甘露醇时，氢摩尔产率可达1.6摩尔。综上所述，菌株AM2即使在盐度条件下也能高产率地从甘露醇中产生氢气。
To achieve more stable bio-hydrogen (bioH2) production from non-food feedstocks, stable feedstock preparations of marine biomass and an efficient bioH2system using marine bacteria under saline conditions are two important key technologies that needed to be developed.Vibrio tritoniusstrain AM2, which was isolated from the gut of a marine invertebrate, was cultured under various conditions in marine broth (at initial 2.25% (w/v) NaCl) supplemented with mannitol, a seaweed carbohydrate, to evaluate its hydrogen production. The maximum molar yield of bioH2was recorded as 1.7 mol H2/mol mannitol at pH 6 and 37 °C. The mannitol-grown cells had higher yields of bioH2than the glucose-grown cells in the pH range 5.5–7.5. Compared to glucose, mannitol might be a better substrate for bioH2production using strain AM2. Fermentation product profiling revealed that strain AM2 might be utilising the formate-hydrogen pathway for bioH2production. Furthermore, strain AM2 was able to produce hydrogen from powdered brown macroalgae containing 31.1% dry weight of mannitol. The molar yield of hydrogen reached 1.6 mol H2/mol mannitol contained in the seaweed feedstock. In conclusion, strain AM2 has the ability to produce hydrogen from mannitol with high yields even under saline conditions.