Clonal propagation of primate offspring by embryo splitting
Clonal propagation of primate offspring by embryo splitting
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DOI：
10.1126/science.287.5451.317
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发表时间：
2000-01-14
期刊：
影响因子：
56.9
通讯作者：
Schatten, GP
中科院分区：
文献类型：
--
作者：
Chan, AWS;Dominko, T;Schatten, GP
目前的克隆策略还没有产生出在细胞核和细胞质成分上都相同的灵长类动物，但这种相同的灵长类动物代表了研究人类疾病的理想模型。在这里，通过分裂期胚胎的卵裂球的分离和重新聚集，产生了遗传上相同的非人类胚胎作为双胞胎和更大的胚胎集。通过分裂107个恒河猴胚胎，在13个胚胎移植后建立了4个妊娠，共产生了368个倍数（31%对53%体外受精对照）。Tetra是一只从四分之一胚胎中克隆出来的健康雌性动物，它的诞生证明了这种方法可以产生活的后代。
Primates that are identical in both nuclear and cytoplasmic components have not been produced by current cloning strategies, yet such identicals represent the ideal model for investigations of human diseases. Here, genetically identical nonhuman embryos were produced as twin and larger sets by separation and reaggregation of blastomeres of cleavage-stage embryos. A total of 368 multiples were created by the splitting of 107 rhesus embryos with four pregnancies established after 13 embryo transfers (31% versus 53% in vitro fertilization controls). The birth of Tetra, a healthy female cloned from a quarter of an embryo, proves that this approach can result in live offspring.