A novel microarray to evaluate stress-related genes in skin: effect of ultraviolet light radiation.
A novel microarray to evaluate stress-related genes in skin: effect of ultraviolet light radiation.
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一种评估皮肤压力相关基因的新型微阵列：紫外线辐射的影响。
DOI：
10.1016/j.gene.2004.06.046
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
Slominski,Andrzej
中科院分区：
文献类型：
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作者：
Pisarchik,Alexander;Wortsman,Jacobo;Slominski,Andrzej
我们使用含有700多个神经内分泌免疫相关基因的微阵列芯片测试了表皮角质形成细胞对紫外线辐射的转录反应。基因表达模式具有非随机和时间依赖性；它包括参与水盐平衡、前列腺素合成、角质细胞分化以及应激效应、细胞因子和金属蛋白酶（MMPs）编码基因的表达增加。相反，编码生长因子及其受体的基因和细胞外基质元件的基因表达减少。这种模式表明，转录反应是协调的，旨在保护表皮屏障功能，预防早期致癌事件和重塑细胞外基质。总之，我们已经开发了一种微阵列芯片，可用于同时测试皮肤神经内分泌免疫功能背景下对皮肤应激源的转录反应。
We tested transcriptional responses to ultraviolet radiation in epidermal keratinocytes using microarray chips containing more than 700 neuroendocrine-immune-related genes. The gene expression pattern was nonrandom and time-dependent; it included increased expression of genes involved in water and salt balance, prostaglandin synthesis, keratinocyte differentiation as well as genes coding for stress effectors, cytokines and metalloproteinases (MMPs). In contrast, expression was decreased for genes coding for growth factors and their receptors and for elements of extracellular matrix. This pattern suggests that transcriptional responses are coordinated and aimed at the preservation of epidermal barrier function, prevention of early carcinogenic events and remodeling of extracellular matrix. In summary, we have developed a microarray chip that can be used for simultaneous testing of transcriptional responses to cutaneous stressors in the context of neuroendocrine-immune functions of the skin.