Protective effect of Fructus Lycii polysaccharides against time and hyperthermia-induced damage in cultured seminiferous epithelium.
Protective effect of Fructus Lycii polysaccharides against time and hyperthermia-induced damage in cultured seminiferous epithelium.
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DOI：
10.1016/s0378-8741(02)00169-1
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发表时间：
2002-10
影响因子：
5.4
通讯作者：
Yan-rong Wang;Hui Zhao;Xinsheng Sheng;Patricia E. Gambino;Brian Costello;K. Bojanowski
中科院分区：
文献类型：
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作者：
Yan-rong Wang;Hui Zhao;Xinsheng Sheng;Patricia E. Gambino;Brian Costello;K. Bojanowski
枸杞子(Lycium barbarum L.)为茄科(Solanaceae)植物，其果实(Luctus Lycii)是我国医家用于治疗不孕不育的药用植物。然而，枸杞促进生育的有效成分和作用机制仍不清楚。在此，我们报道了枸杞子多糖(FLPS)在体外抑制时间和高温诱导的小鼠生精上皮结构损伤。此外，我们还发现，在常温和高温培养条件下，Flps都延迟了该系统的细胞凋亡。氧化应激被认为是高热睾丸结构退化和细胞凋亡的主要原因，因此，FLPS的保护作用可能涉及抗氧化作用机制。为了验证这一假设，我们测试了Flps对紫外光诱导的脂质过氧化和自由基对细胞色素c还原的影响。我们发现Flps对这两种反应都有很强的抑制作用。综上所述，这些结果证实了FLPS对时间和高温诱导的体外睾丸变性的保护作用，揭示了这种保护作用的潜在作用机制，为该植物的传统使用提供了科学依据。
Lycium barbarum L. (Solanaceae) is a Chinese medicinal plant whose fruits (Fructus Lycii) are used by Chinese physicians for treatment of infertility. However, the active ingredients and the mechanism of action underlying Lycium's fertility-facilitating effects remain unknown. Here we report that Fructus Lycii polysaccharides (FLPS) inhibit time- and hyperthermia-induced structural damage in murine seminiferous epithelium, in vitro. Moreover, we found that FLPS delayed apoptosis in this system, both at normothermic and hyperthermic culture conditions. Oxidative stress was reported to be a major cause of structural degradation and apoptosis in hyperthermic testes, and thus the protective effect of FLPS could implicate an antioxidant mechanism of action. To test this hypothesis we assayed the effect of FLPS on ultraviolet light-induced lipid peroxidation, and cytochrome c reduction by free radicals. We found that FLPS is a potent inhibitor of both of these reactions. Together, these results demonstrate the protective effect of FLPS on time- and hyperthermia-induced testicular degeneration in vitro, indicate the potential mechanism of action for this protective effect, and provide a scientific basis for the traditional use of this plant.