Antagonistic regulation of the gibberellic acid response during stem growth in rice
Antagonistic regulation of the gibberellic acid response during stem growth in rice
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DOI：
10.1038/s41586-020-2501-8
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发表时间：
2020-07-15
期刊：
影响因子：
64.8
通讯作者：
Ashikari, Motoyuki
中科院分区：
文献类型：
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作者：
Nagai, Keisuke;Mori, Yoshinao;Ashikari, Motoyuki
植物的大小很大程度上取决于茎的生长。赤霉酸刺激茎伸长（1-3）。在这里，我们表明，水稻节间茎伸长是由一个“加速器”和“减速器”在音乐会与赤霉素拮抗调节。一个基因的表达，我们命名为加速器节间伸长1（ACE 1），它编码一种蛋白质的未知功能，赋予细胞分裂的能力，导致节间伸长的存在下，赤霉酸的中间分生组织区域的细胞。相反，上调编码锌指转录因子的节间伸长抑制因子1（DEC 1）抑制节间伸长，而下调DEC 1则允许节间伸长。我们还表明，由ACE 1和DEC 1介导的节间伸长机制在禾本科植物中是保守的。此外，遗传多样性的分析表明，ACE 1和DEC 1的突变在历史上有助于选择较短的植物在驯化群体的水稻，以增加其抗倒伏性，和较高的植物在野生品种的水稻适应生长在深水中。这些拮抗调节因子的鉴定增强了我们对赤霉酸反应作为调节节间伸长和环境适应性的额外机制的理解，超越了生物合成和赤霉酸信号转导。
The size of plants is largely determined by growth of the stem. Stem elongation is stimulated by gibberellic acid(1-3). Here we show that internode stem elongation in rice is regulated antagonistically by an 'accelerator' and a 'decelerator' in concert with gibberellic acid. Expression of a gene we nameACCELERATOR OF INTERNODE ELONGATION 1(ACE1), which encodes a protein of unknown function, confers cells of the intercalary meristematic region with the competence for cell division, leading to internode elongation in the presence of gibberellic acid. By contrast, upregulation ofDECELERATOR OF INTERNODE ELONGATION 1(DEC1), which encodes a zinc-finger transcription factor, suppresses internode elongation, whereas downregulation ofDEC1allows internode elongation. We also show that the mechanism of internode elongation that is mediated byACE1andDEC1is conserved in the Gramineae family. Furthermore, an analysis of genetic diversity suggests that mutations inACE1andDEC1have historically contributed to the selection of shorter plants in domesticated populations of rice to increase their resistance to lodging, and of taller plants in wild species of rice for adaptation to growth in deep water. Our identification of these antagonistic regulatory factors enhances our understanding of the gibberellic acid response as an additional mechanism that regulates internode elongation and environmental fitness, beyond biosynthesis and gibberellic acid signal transduction.