Molecular Basis of Haptoglobin and Hemoglobin Complex Formation and Protection against Oxidative Stress and Damage
Molecular Basis of Haptoglobin and Hemoglobin Complex Formation and Protection against Oxidative Stress and Damage
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触珠蛋白和血红蛋白复合物形成的分子基础以及针对氧化应激和损伤的保护
DOI：
10.1142/9789814472869_0007
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发表时间：
2013
期刊：
影响因子：
5.3
通讯作者：
A. Alayash
中科院分区：
文献类型：
--
作者：
Yiping Jia;A. Alayash
触珠蛋白 (Hp) 是一种血浆蛋白，可清除循环中的细胞游离血红蛋白 (Hb)，并减少 Hb 形成的有害氧化物质。在人血浆中可以发现三种 Hp 表型，即 Hp 1-1、Hp 2-1 和 Hp 2-2；所有这些都已被证明能够与配体 Hb 紧密但可逆地结合。快速动力学研究检查了 Hp 与 Hb 以及分离的 Hb 亚基的结合特征。在这些研究中，结合 Hb 配体反应性和氧化还原特性的改变，详细阐述了 Hp 和 Hb 复合物形成的分子机制。最近提出 Hp 抗氧化活性可减轻由无细胞 Hb 和基于 Hb 的氧载体 (HBOC) 引起的氧化应激和细胞/组织损伤。本章综述了与游离和修饰的 Hb 形成的 Hp 复合物的理化特性，以及该复合物的酶活性在防止 Hb 氧化毒性和维持组织稳态方面的潜在用途。
Haptoglobin (Hp) is a plasma protein that clears cell free hemoglobin (Hb) from circulation and reduces the formation of harmful oxidative species by Hb. Three phenotypes of Hp can be found in human plasma, and these are Hp 1–1, Hp 2–1, and Hp 2–2; all have been shown to tightly but reversibly bind with liganded Hb. Rapid kinetic studies have examined the binding characteristics of Hp with Hb, as well as with isolated Hb subunits. The molecular mechanisms of Hp and Hb complex formation were elaborated in relation to the altered Hb ligand reactivities and redox properties in these studies. The Hp antioxidant activity was recently proposed to alleviate oxidative stress and cell/tissue damage caused by cell free Hb, and Hb-based oxygen carriers (HBOCs). In this chapter, the physicochemical characteristics of the Hp complex formation with free and modified Hbs and the potential utility of the enzymatic activities of the complex in protecting against Hb oxidative toxicity and in maintaining tissue homeostasis are reviewed.
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Valette,I;Waks,M;Wejman,JC;Arcoleo,JP;Greer,J
通讯作者：
Greer,J
使用触珠蛋白结合研究人氧合血红蛋白β四聚体解离。
DOI：
10.1016/s0006-291x(88)80860-x
发表时间：
1988
影响因子：
3.1
作者：
Michalski,LA;McDonald,MJ
通讯作者：
McDonald,MJ
影响因子：
20.3
作者：
Melamed-Frank, M;Lache, O;Levy, AP
通讯作者：
Levy, AP