Hypoionic shock treatment enables aminoglycosides antibiotics to eradicate bacterial persisters.
Hypoionic shock treatment enables aminoglycosides antibiotics to eradicate bacterial persisters.
复制标题
低离子休克治疗使氨基糖苷类抗生素能够根除细菌持续存在
DOI：
10.1038/srep14247
复制
发表时间：
2015-10-05
影响因子：
4.6
通讯作者：
Chang Z
中科院分区：
文献类型：
--
作者：
Jiafeng L;Fu X;Chang Z
细菌持续存在者通常被认为是对抗生素耐受的休眠细胞，是反复感染和抗生素耐药病原体出现的重要来源。临床根除致病性持久性是非常需要的，但非常困难，主要是由于抗生素摄取的大幅减少以及药物靶点的非活性状态。在这里，我们报告了在低离子休克（例如纯水处理）下，即使不到一分钟，氨基糖苷类抗生素也可以有效地根除细菌持续存在（正常生长的细胞）。这种对氨基糖苷的低离子冲击增强效应与质子动力无关，显然是通过促进氨基糖苷的进入来实现的，推测是通过机械敏感离子通道。我们的发现可能提供一种简单而有力的策略来根除病原体。
Bacterial persisters, usually being considered as dormant cells that are tolerant to antibiotics, are an important source for recurrent infection and emergence of antibiotic resistant pathogens. Clinical eradication of pathogenic persisters is highly desired but greatly difficult mainly due to the substantial reduction in antibiotics uptake as well as the non-active state of the drug targets. Here we report that bacterial persisters (normal growing cells as well) can be effectively eradicated by aminoglycoside antibiotics upon hypoionic shock (e.g. pure water treatment) even for less than one minute. Such hypoionic shock potentiation effect on aminoglycosides is proton motive force-independent and is apparently achieved by promoting the entrance of aminoglycosides, speculatively through the mechanosensitive ion channels. Our revelations may provide a simple and powerful strategy to eradicate pathogen persisters.