INFORMATION-CONTENT IN THE CIRCULAR-DICHROISM OF PROTEINS
INFORMATION-CONTENT IN THE CIRCULAR-DICHROISM OF PROTEINS
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DOI：
10.1021/bi00508a007
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发表时间：
1981-01-01
期刊：
影响因子：
2.9
通讯作者：
JOHNSON, WC
中科院分区：
文献类型：
--
作者：
HENNESSEY, JP;JOHNSON, WC
提出了一种从蛋白质的圆二色谱(CD)预测蛋白质二级结构的方法。考虑了八种类型的二级结构：螺旋、平行和反平行β。链；类型I、II和III.Beta。转身；所有其他的.测试版。转弯组合；以及其他结构。该方法是基于对已知二级结构蛋白质的CD谱进行正交基CD谱的数学计算。需要5个基圆二色谱才能重建延伸到真空紫外区的16个原始蛋白质圆二色谱至178 nm。因此，人们可以期望从蛋白质的CD光谱中提取5个独立的信息。每个基本CD谱对应于一种已知的二级结构混合物，因此重建蛋白质CD谱的系数也可用于预测二级结构。当同样的方法应用于蛋白质二级结构而不是CD时，只需5个碱基二级结构向量即可重构原始蛋白质二级结构向量。因此，在所研究的蛋白质中有5个独立的超结构(由标准二级结构的混合物组成)。蛋白质的CD光谱显然有足够的信息来预测所有类型的二级结构。CD分析与X-射线数据相比具有较好的可比性。
A method is presented for predicting the secondary structure of a protein from its circular dichroism (CD) spectrum. Eight types of secondary structure are considered: helix; parallel and antiparallel .beta. strand; types I, II and III .beta. turn; all other .beta. turns combined; and other structures. The method is based on mathematical calculation of orthogonal basis CD spectra from the CD spectra of proteins with known secondary structure. Five basis CD spectra are needed to reconstruct the 16 original protein CD spectra that extend into the vacuum UV region to 178 nm. Thus, one can expect to extract 5 independent pieces of information from the CD spectrum of a protein. Each basis CD spectrum corresponds to a known mixture of secondary structures so that the coefficients that reconstruct the protein CD spectrum can also be used to predict secondary structure. When the same method is applied to protein secondary structure rather than CD, only 5 basis secondary structure vectors are needed to reconstruct the original protein secondary structure vectors. Thus, there are 5 independent superstructures (consisting of a mixture of standard secondary structures) in the proteins studied. There is apparently enough information in the CD spectrum of a protein to predict all types of secondary structure. CD analyses compare favorably with the X-ray data.