Insulin stimulates ecdysteroid production through a conserved signaling cascade in the mosquito Aedes aegypti
Insulin stimulates ecdysteroid production through a conserved signaling cascade in the mosquito Aedes aegypti
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DOI：
10.1016/s0965-1748(99)00084-3
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发表时间：
1999-10-01
影响因子：
3.8
通讯作者：
Brown, MR
中科院分区：
文献类型：
--
作者：
Riehle, MA;Brown, MR
测试了哺乳动物细胞中胰岛素信号级联的选择性激活剂和抑制剂对埃及伊蚊卵巢胰岛素刺激的类固醇生成的影响。牛胰岛素在1.7 μ M至85 μ M的浓度范围内刺激体外蜕皮甾体生成。过钒酸盐，酪氨酸激酶磷酸酶的抑制剂，刺激蜕皮激素的生产在浓度为250 μ M至1 mM。Okidaic酸，丝氨酸/苏氨酸磷酸酶抑制剂，刺激类固醇的ED 50为77.39 nM。酪氨酸激酶活性的选择性抑制剂HNMPA-（AM（3））抑制蜕皮甾类的产生，IC 50为14.2 μ M。磷脂酰肌醇3-激酶的两种选择性抑制剂wortmannin和LY 294002在低浓度下抑制蜕皮类固醇的产生（IC 50分别为1.6 nM和30 nM）。这些浓度与哺乳动物细胞中抑制胰岛素作用的浓度相似。有丝分裂原活化蛋白激酶的选择性抑制剂，PD 098059，甚至高达100 μ M的蜕皮激素的生产没有影响。因此，胰岛素刺激卵巢在体外产生蜕皮类固醇似乎是由蚊子胰岛素受体的酪氨酸激酶活性和涉及磷脂酰肌醇3-激酶和蛋白激酶B的信号级联控制的。(C)1999爱思唯尔科技有限公司。保留所有权利。
Selective activators and inhibitors of insulin signaling cascades in mammalian cells were tested for their effects on insulin stimulated steroidogenesis by ovaries of Aedes aegypti. Bovine insulin in the concentration range of 1.7 mu M to 85 mu M stimulated ecdysteroidogenesis in vitro. Pervanadate, an inhibitor of tyrosine kinase phosphatase, stimulated ecdysteroid production at concentrations of 250 mu M to 1 mM. Okidaic acid, a serine/threonine phosphatase inhibitor, stimulated steroidogenesis with an ED50 of 77.39 nM. A selective inhibitor of tyrosine kinase activity, HNMPA-(AM(3)), inhibited ecdysteroid production with an IC50 of 14.2 mu M. Two selective inhibitors of phosphatidylinositol 3-kinase, wortmannin and LY294002, inhibited ecdysteroid production at low concentrations (IC50 = 1.6 nM and 30 nM, respectively). These concentrations are similar to those inhibiting insulin action in mammalian cells. A selective inhibitor of mitogen-activated protein kinase, PD098059, had no effect on ecdysteroid production even up to 100 mu M. Thus, insulin stimulation of ecdysteroid production by ovaries in vitro appears to be controlled by the tyrosine kinase activity of the mosquito insulin receptor and the signaling cascade involving phosphatidylinositol 3-kinase and protein kinase B. (C) 1999 Elsevier Science Ltd. All rights reserved.