Development to term of chimaeras between diploid parthenogenetic and fertilised embryos
Development to term of chimaeras between diploid parthenogenetic and fertilised embryos
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二倍体单性生殖和受精胚胎之间嵌合体的发育
DOI：
10.1038/270601a0
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发表时间：
1977
期刊：
影响因子：
64.8
通讯作者：
Matthew H. Kaufman
中科院分区：
文献类型：
--
作者：
M. Surani;S. Barton;Matthew H. Kaufman
孤雌生殖小鼠胚胎具有广泛的细胞增殖和分化为各种细胞类型1-3的潜力。但只有当孤雌生殖胚胎被移植到宫外1，以及自发发生的卵巢畸胎瘤和孤雌生殖起源的畸胎癌2，3时，这种潜力才能实现。哺乳动物孤雌胚胎在子宫内的发育受到限制，没有确凿的证据表明它们可以发育到4，5期。有人提出了几种假说来解释它们发育不良的原因。例如，有害的隐性基因可能会影响其细胞的活性，可能是因为它们广泛的纯合2，5，或由于细胞相互作用的异常而导致的无组织生长和有限的寿命5。但发生在宫外的孤雌生殖来源细胞的广泛细胞增殖和分化与这些解释并不完全一致，表明孤雌生殖可能具有相对稳定的遗传结构。事实上，这些研究强调了细胞环境对细胞分化的重要性，在这种情况下，细胞分化是由宫外宿主组织提供的。如果环境对细胞分化至关重要，那么孤雌生殖细胞应该能够与来自受精胚胎的细胞形成嵌合体，这是有先例的。畸胎癌细胞7、8和携带已知致死等位基因9、10的细胞在与正常胚胎的细胞聚集时可以发育成可存活的嵌合体。以前试图实现孤雌生殖和受精胚胎之间的聚合嵌合体的发育，显然是不成功的11。我们已经将来自二倍体孤雌胚胎的内细胞团(ICM)引入到完整的受精小鼠囊胚中，我们在这里报告了嵌合体到足月的发育。
PARTHENOGENETIC mouse embryos have the potential for extensive cellular proliferation as well as differentiation into various cell types1–3. But this potential has been realised only when parthenogenetic embryos have been transferred to extrauterine sites1, and in spontaneously occurring ovarian teratomas and teratocarcinomas of parthenogenetic origin2,3. The development of mammalian parthenogenetic embryos in utero is restricted, with no conclusive evidence that they can develop to term4,5. Several hypotheses have been proposed to account for their poor development. For example, deleterious recessive genes may affect the viability of their cells, possibly because of their extensive homozygosity2,5, or disorganised growth and limited life span may result from anomalies of cellular interactions5. But the extensive cellular proliferation and differentiation of parthenogenetically derived cells which occurs in extrauterine sites is not entirely consistent with these explanations, and indicates that parthenogenones probably have a relatively stable genetic constitution. Indeed, these studies stress the likely importance of cellular environment for cytodifferentiation, provided in this instance by the extrauterine host tissue. There is a precedent for supposing that if the environment is critical for cytodifferentiation, parthenogenetic cells should be able to form chimaeras with cells derived from fertilised embryos6. Teratocarcinoma cells7,8 and cells carrying known lethal alleles9,10 can develop into viable chimaeras when aggregated with cells from normal embryos. Previous attempts to achieve development to term of aggregation chimaeras between parthenogenetic and fertilised embryos were apparently unsuccessful11. We have introduced inner cell masses (ICMs) from diploid ,parthenogenetic embryos into intact fertilised mouse blastocysts, and we report here the development of a chimaera to term.
小鼠畸胎癌发生和自发孤雌生殖。
影响因子：
--
作者：
Stevens,LC
通讯作者：
Stevens,LC