CHOLINERGIC PROJECTIONS TO THE SUBSTANTIA NIGRA FROM THE PEDUNCULOPONTINE AND LATERODORSAL TEGMENTAL NUCLEI
CHOLINERGIC PROJECTIONS TO THE SUBSTANTIA NIGRA FROM THE PEDUNCULOPONTINE AND LATERODORSAL TEGMENTAL NUCLEI
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DOI：
10.1016/0306-4522(89)90008-0
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发表时间：
1989-01-01
期刊：
影响因子：
3.3
通讯作者：
BUTCHER, LL
中科院分区：
文献类型：
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作者：
GOULD, E;WOOLF, NJ;BUTCHER, LL
采用高灵敏度的逆行和顺行神经束追踪方法，结合胆碱乙酰转移酶免疫组织化学方法，研究了大鼠黑质致密部和网状部的胆碱能神经支配。荧光示踪剂真蓝，碘化丙啶，或荧光金被优先注入任一黑质亚核。胆碱乙酰转移酶和逆行示踪剂阳性细胞被发现在pedunculopontine和laterodorsal被盖核，虽然相当多的双标记胞体观察到前者比后者组成部分的脑桥脑被盖胆碱能复合体。大约2-3倍以上的胆碱能细胞被标记在peduculopontine和laterodorsal被盖核时，示踪剂注射集中在紧凑的黑质细分时，约相同大小的输液完全局限于网状部分。注入顺行运输示踪菜豆白细胞凝集素到脑桥中脑被盖胆碱能复合物导致的吸收和运输的标签，黑质亚核，和一些菜豆白细胞凝集素积累的胞体和近端的过程也表现出胆碱乙酰转移酶样免疫反应。菜豆凝集素标记的实体在黑质中表现出终端样的配置文件，这让人想起的模式，黑质胆碱乙酰转移酶阳性斑点证明，通过使用硫酸镍铵增强的最终反应产物在抗生物素蛋白-生物素程序的化学。这些观察结果有力地支持了这一论点，即桥脑被盖胆碱能复合体是胆碱能投射到大鼠黑质的致密和网状部分的主要来源。
The cholinergic innervation of the compact and reticular parts of the substantia nigra in the rat was investigated by use of highly sensitive retrograde and anterograde tract-tracing methods in combination with choline acetyltransferase immunohistochemistry. The fluorescent tracers True Blue, propidium iodide, or fluorogold were infused preferentially into either nigral subnucleus. Cells positive for choline acetyltransferase and retrograde tracer were found in both the pedunculopontine and laterodorsal tegmental nuclei, although considerably more double-labeled somata were observed in the former than in the latter component of the pontomesencephalotegmental cholinergic complex. Approximately 2-3 times more cholinergic cells were labeled in the peduculopontine and laterodorsal tegmental nuclei when tracer injections were centered in the compact nigral subdivision than when infusions of about the same size were confined totally to the reticular part. Infusions of the anterogradely transported tracer Phaseolus vulgaris leucoagglutinin into the pontomesencephalotegmental cholinergic complex resulted in uptake and transport of that label to both nigral subnuclei, and some of the Phaseolus vulgaris leucoagglutinin-accumulating somata and proximal processes also demonstrated choline acetyltransferase-like immunoreactivity. The Phaseolus vulgaris agglutinin-labeled entities in the substantia nigra exhibited terminal-like profiles that were reminiscent of the pattern of nigral choline acetyltransferase-positive puncta demonstrated immunohistochemically by use of nickel ammonium sulfate enhancement of the final reaction product in the avidin-biotin procedure. These observations strongly support the contention that the pontomesencephalotegmental cholinergic complex is the major source of cholinergic projections to both the compact and reticular portions of the rat substantia nigra.