ANTIBODIES FROM PATIENTS WITH CONNECTIVE-TISSUE DISEASES BIND SPECIFIC SUBSETS OF CELLULAR RNA-PROTEIN PARTICLES
ANTIBODIES FROM PATIENTS WITH CONNECTIVE-TISSUE DISEASES BIND SPECIFIC SUBSETS OF CELLULAR RNA-PROTEIN PARTICLES
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DOI：
10.1172/jci110587
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发表时间：
1982-01-01
影响因子：
15.9
通讯作者：
STEITZ, JA
中科院分区：
文献类型：
--
作者：
HARDIN, JA;RAHN, DR;STEITZ, JA
对260例抗核抗体阳性患者血清中的RNA抗原进行了鉴定。49例患者中，大多数为系统性红斑狼疮或干燥S综合征患者，其抗体可单独或组合地沉淀先前确定的RNP、Sm、Ro和La抗原。这些抗原分布在小的核或细胞质RNA-蛋白质颗粒的离散集合上，表现出许多抗原相互关系。一位患者S的血清识别出了一种新的含有小核糖核酸的颗粒，命名为Th；它还沉淀了Ro复合体。其他系统性红斑狼疮、乙肝病毒感染、幼年类风湿性关节炎、肌炎和类风湿性关节炎患者的抗体中有rRNA和tRNA的特定亚群。1例患者“S”血清中含有一种可沉淀核糖体的单抗Ig G。这些抗体中的大多数确定了至少由蛋白质部分组成的抗原决定簇。结合特定细胞RNA的蛋白质的特异性可能解释了风湿病患者的抗体在RNA亚群中区分的精确度。这种血清应该是有用的探针，以研究不同的RNA和RNA-蛋白质复合体在细胞代谢中所起的特定作用。
The RNA-containing antigens precipitated by sera from 260 patients with positive antinuclear antibodies were characterized. Forty-nine individuals, most of whom had systemic lupus erythromatosus or Sjogren''s syndrome, possessed antibodies that precipitated the previously identified RNP, Sm, Ro and La antigens singly or in combinations. These antigens, which are located on discrete sets of small nuclear or cytoplasmic RNA-protein particles, exhibited a number of antigenic interrelationships. One patient''s serum recognized a new particle containing a small RNA which was designated Th; it also precipitated the Ro complexes. Other patients with systemic lupus erythematosus, hepatitis B virus infection, juvenile rheumatoid arthritis, myositis and rheumatoid arthritis had antibodies that precipitated specific subsets of rRNA and tRNA. One patient''s serum contained a monoclonal IgG that precipitated ribosomes. Most of these antibodies identified antigenic determinants constituted at least in part of protein. The specificity of the proteins bound to particular cellular RNA probably explains the exquisite precision with which antibodies from rheumatic disease patients discriminate among RNA subsets. Such sera should be useful probes for investigating specific roles that different RNA and RNA-protein complexes play in cellular metabolism.