Polydatin attenuates cadmium-induced oxidative stress via stimulating SOD activity and regulating mitochondrial function in Musca domestica larvae
Polydatin attenuates cadmium-induced oxidative stress via stimulating SOD activity and regulating mitochondrial function in Musca domestica larvae
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虎杖甙通过刺激家蝇幼虫中 SOD 活性和调节线粒体功能来减轻镉诱导的氧化应激
DOI：
10.1016/j.chemosphere.2020.126009
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发表时间：
2020
期刊：
影响因子：
8.8
通讯作者：
Liu Fengsong
中科院分区：
文献类型：
--
作者：
Zhang Yuming;Li Yajing;Feng Qin;Shao Menghua;Yuan Fengyu;Liu Fengsong
随着电镀和冶金工业的发展，镉是一种广泛存在的环境污染物。Cd可被生物体通过食物链富集，造成环境问题扩大，并对人类健康构成威胁。虎杖素是一种从虎杖中提取的天然二苯乙烯类化合物，对氧化损伤有明显的治疗作用。研究了PD对Cd对家蝇幼虫氧化损伤的保护作用。分别暴露于Cd和/或PD环境下，观察其氧化应激状态、线粒体活性、氧化磷酸化效率和存活率。Cd暴露使家蝇幼虫体内丙二醛（MDA）、活性氧（ROS）和8-羟基-2-脱氧鸟苷（8-oxoG）显著升高，导致线粒体功能障碍，成活率下降。有趣的是，PD预处理对cd暴露的幼虫具有明显的线粒体保护作用，表现为MDA、ROS和8-oxoG水平降低，超氧化物歧化酶（SOD）活性、线粒体电子传递链活性、线粒体膜电位活性以及呼吸控制率升高。上述结果表明，PD可通过维持氧化还原平衡、刺激SOD活性、调节线粒体活性等途径减轻cd诱导的家蝇损伤。PD是一种天然多酚类化合物，可作为缓解Cd损伤的潜在候选化合物。
Cadmium (Cd) is a widespread environment contaminant due to the development of electroplating and metallurgical industry. Cd can be enriched by organisms via food chain, causing the enlarged environmental problems and posing threats to the health of humans. Polydatin (PD), a natural stilbenoid compound derived fromPolygonum cuspidatum, shows pronouncedly curative effect on oxidative damage. In this work, the protective effects of PD on oxidative damage induced by Cd inMusca domestica(housefly) larvae were evaluated. The larvae were exposed to Cd and/or PD, subsequently, the oxidative stress status, mitochondria activity, oxidative phosphorylation efficiency, and survival rate were assessed. Cd exposure generated significant increases of malondialdehyde (MDA), reactive oxygen species (ROS) and 8-hydroxy-2-deoxyguanosine (8-oxoG) in the housefly larvae, causing mitochondrial dysfunction and survival rate decline. Interestingly, pretreatment with PD exhibited obviously mitochondrial protective effects in the Cd-exposed larvae, as evidenced by reduced MDA, ROS and 8-oxoG levels, and increased activities of superoxide dismutase (SOD), mitochondrial electron transfer chain, and mitochondrial membrane potential, as well as respiratory control ratio. These results suggested that PD could attenuate Cd-induced damage via maintaining redox balance, stimulating SOD activity, and regulating mitochondria activity in housefly larvae. As a natural polyphenolic chemical, PD can act as a potential candidate compounds to relieve Cd injury.