Long-term exposure of CdTe quantum dots on PC12 cellular activity and the determination of optimum non-toxic concentrations for biological use.
Long-term exposure of CdTe quantum dots on PC12 cellular activity and the determination of optimum non-toxic concentrations for biological use.
复制标题
DOI：
10.1186/1477-3155-8-7
复制
发表时间：
2010-03-25
影响因子：
10.2
通讯作者：
Rochev Y
中科院分区：
文献类型：
--
作者：
Prasad BR;Nikolskaya N;Connolly D;Smith TJ;Byrne SJ;Gérard VA;Gun'ko YK;Rochev Y
量子点（QD）的独特和可调的光子特性使它们成为成像生物实体的潜在有用工具。然而，量子点虽然有吸引力的诊断和治疗工具，有一个主要的缺点，由于其固有的细胞毒性性质。细胞的相互作用，摄取和由此产生的毒性影响的CdTe量子点（明胶化和非明胶化巯基乙酸（TGA）封端）已与嗜铬细胞瘤12（PC 12）细胞进行了研究。结合通过共聚焦显微镜进行的分析，探索QD -细胞相互作用，因为QD浓度在延长的（长达72小时）共孵育时间内变化。结合本研究，还进行了细胞活力、DNA定量和细胞增殖测定，以比较和对比导致细胞应激和最终死亡的各种因素。将巯基乙酸（TGA）稳定的CdTe QD（凝胶和非凝胶）与PC 12细胞共孵育，并研究它们的存在如何影响细胞行为和功能。随着QD浓度在长达72小时的共孵育期间变化，分析细胞形态。发现QD是优异的荧光团，照亮细胞的细胞质，并且在~10-9 M的浓度下没有观察到有害作用。利用三种测定来探索不同浓度和孵育时间的QD的存在如何影响单个细胞功能（活力、DNA定量和增殖）。发现细胞反应不仅是浓度依赖性的，而且还受到量子点表面环境的影响。表面上的明胶封端充当对有毒原子泄漏的屏障，从而减少QD的负面影响。这项研究表明，在正确的条件下，量子点可以常规用于PC 12细胞的成像，副作用最小。我们已经发现，PC 12细胞对增加的QD浓度范围高度敏感，而明胶涂层在较高QD浓度下充当增强毒性的屏障。
The unique and tuneable photonic properties of Quantum Dots (QDs) have made them potentially useful tools for imaging biological entities. However, QDs though attractive diagnostic and therapeutic tools, have a major disadvantage due to their inherent cytotoxic nature. The cellular interaction, uptake and resultant toxic influence of CdTe QDs (gelatinised and non-gelatinised Thioglycolic acid (TGA) capped) have been investigated with pheochromocytoma 12 (PC12) cells. In conjunction to their analysis by confocal microscopy, the QD - cell interplay was explored as the QD concentrations were varied over extended (up to 72 hours) co-incubation times. Coupled to this investigation, cell viability, DNA quantification and cell proliferation assays were also performed to compare and contrast the various factors leading to cell stress and ultimately death. Thioglycolic acid (TGA) stabilised CdTe QDs (gel and non - gel) were co-incubated with PC12 cells and investigated as to how their presence influenced cell behaviour and function. Cell morphology was analysed as the QD concentrations were varied over co-incubations up to 72 hours. The QDs were found to be excellent fluorophores, illuminating the cytoplasm of the cells and no deleterious effects were witnessed at concentrations of ~10-9 M. Three assays were utilised to probe how individual cell functions (viability, DNA quantification and proliferation) were affected by the presence of the QDs at various concentrations and incubation times. Cell response was found to not only be concentration dependant but also influenced by the surface environment of the QDs. Gelatine capping on the surface acts as a barrier towards the leaking of toxic atoms, thus reducing the negative impact of the QDs. This study has shown that under the correct conditions, QDs can be routinely used for the imaging of PC12 cells with minimal adverse effects. We have found that PC12 cells are highly susceptible to an increased concentration range of the QDs, while the gelatine coating acts as a barrier towards enhanced toxicity at higher QD concentrations.