Preparation of electro-spun konjac glucomannan fabric with entrapped DNA and dynamics of adsorption of acridine orange for carcinogen removal application
Preparation of electro-spun konjac glucomannan fabric with entrapped DNA and dynamics of adsorption of acridine orange for carcinogen removal application
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包埋DNA的电纺魔芋葡甘聚糖织物的制备及吖啶橙吸附动力学去除致癌物的应用
DOI：
10.1007/s10450-022-00357-9
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发表时间：
2022
期刊：
影响因子：
3.3
通讯作者：
Dobashi Toshiaki
中科院分区：
文献类型：
--
作者：
Yamaguchi Gaku;Yoshiba Kazuto;Kawada Sara;Sato Ryosuke;Nagai Daisuke;Maki Yasuyuki;Yamamoto Takao;Tanaka Susumu;Chu Benjamin;Dobashi Toshiaki
采用静电纺丝法制备了含双链DNA分子的魔芋葡甘聚糖（KGM）非织造布，并将其与1，6-二异氰酸酯己烷交联。用唯象理论对模型致癌物吖啶橙子（AO）在织物上的吸附进行了测量和分析。织物对AO的吸附符合一个混合方程，该方程由初期的扩散限制方程和后期的化学计量方程组成。所有的观测吸附数据进行了分析的理论公式，一个时间常数的初始阶段，和三个参数，包括时间常数，吸附的AO在平衡的数目和超调因子的后期阶段。初始阶段的时间常数与AO浓度成正比，与吸附织物重量的平方成反比。有人还发现，在平衡吸附AO的数量仅由织物中的DNA的量，而时间常数为后期可以变得更大的织物中的DNA的浓度，f，变得更大。研究结果可为设计一种有效的吸附方法以去除织物中的双链DNA分子中的致癌物提供指导。
A nonwoven fabric of konjac glucomannan (KGM) containing double stranded DNA molecules was fabricated by using the electro-spinning method and followed by crosslinking of KGM with 1,6-diisocyanatohexane. The adsorption of acridine orange (AO), as a model carcinogen, to the fabric was measured and analyzed by using a phenomenological theory. The fabric adsorbed AO quantitatively according to a hybrid equation, consisting of a diffusion-limited equation during the initial stage and a stoichiometric equation at the later stage. All the observed adsorption data were analyzed by the theoretical formulas with one time constant for the initial stage, and three parameters containing time constant, number of adsorbed AO at equilibrium and the overshoot factor for the later stage. The time constant for the initial stage increased proportionally with AO concentration and inversely with the square of weight of adsorbent fabric. It was also found that the number of adsorbed AO at equilibrium was determined only by the amount of DNA in the fabric, whereas the time constant for the later stage could become larger as the concentration of DNA in the fabric,f, became larger. The results can be used as a guideline to design an efficient adsorption procedure for the removal of carcinogens by the double-stranded DNA molecules in the fabric.
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DOI：
--
发表时间：
1965
期刊：
影响因子：
--
作者：
M. Lamm;D. Neville
通讯作者：
D. Neville
影响因子：
--
作者：
Bin Li;B. Xie
通讯作者：
Bin Li;B. Xie
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DOI：
--
发表时间：
2002
期刊：
Chem.European J. 8(6)
影响因子：
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作者：
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DOI：
10.1093/oxfordjournals.jbchem.a129839
发表时间：
1972
期刊：
Journal of Biochemistry (Tokyo)
影响因子：
--
作者：
M. Sakoda;K. Hiromi;K. Akasaka
通讯作者：
K. Akasaka
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DOI：
--
发表时间：
2007
期刊：
Trans. MRS-J 32
影响因子：
--
作者：
Y. Maki;K. Furusawa;M. Wakamatsu;T. Yamamoto. T. Dobashi
通讯作者：
T. Yamamoto. T. Dobashi