EFFECTS OF CYTOKINES ON INTRACELLULAR GROWTH OF BRUCELLA-ABORTUS
EFFECTS OF CYTOKINES ON INTRACELLULAR GROWTH OF BRUCELLA-ABORTUS
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DOI：
10.1128/iai.61.1.124-134.1993
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发表时间：
1993-01-01
影响因子：
3.1
通讯作者：
BALDWIN, CL
中科院分区：
文献类型：
--
作者：
JIANG, XS;BALDWIN, CL
检测了白细胞介素1 α（IL-1 α）、IL-2、IL-4、IL-6、γ干扰素（IFN-γ）、肿瘤坏死因子α（TNF-α）和粒细胞巨噬细胞集落刺激因子（GM-CSF）改变BALB/c J774A.1小鼠巨噬细胞中流产布鲁氏菌生长的能力。IL-1 α、IL-4、IL-6、肿瘤坏死因子α和粒细胞-巨噬细胞-集落刺激因子对无毒力B的生长没有一致或显著的影响。流产菌株19。相反，向巨噬细胞培养物中加入100 U/ml的IFN-γ或IL-2导致细胞内细菌数量显著减少，这不是由于感染率降低所致。使用IL-2时，减少通常仅在感染后的前24小时内最明显，而使用IFN-γ时的抑制在整个48小时的培养期内都是明显的。向巨噬细胞培养物中加入IL-2或IFN-γ也导致毒性B的细胞内CFU减少。牛流产杆菌菌株2308和减毒粗糙突变体B。牛流产杆菌菌株RB 51。细胞内生长的抑制并没有增加细胞因子的组合。用IFN-γ和IL-2进行的其他研究表明，它们可以介导对B细胞内生长的抑制。流产在居民和巯基乙酸盐肉汤诱导的BALB/c腹腔巨噬细胞和脾巨噬细胞。IFN-γ在巨噬细胞感染后加入时也能抑制细菌生长，尽管其抗布鲁氏菌作用的程度低于在感染前加入时。此外，最大的抑制作用持续只有当IFN-γ仍然在感染后的巨噬细胞的培养物。
Interleukin 1alpha (IL-1alpha) IL-2, IL-4, IL-6, gamma interferon (IFN-gamma), tumor necrosis factor alpha (TNF-alpha), and granulocyte macrophage colony-stimulating factor (GM-CSF) were tested for their abilities to alter the growth of Brucella abortus in BALB/c J774A.1 murine macrophages. IL-1alpha, IL-4, [L-6, tumor necrosis factor alpha, and granulocyte macrophage--colony-stimulating factor had no consistent or significant effect on the growth of the avirulent B. abortus strain 19. In contrast, the addition of either IFN-gamma or IL-2 at 100 U/ml to the macrophage cultures resulted in a significant reduction in the number of intracellular bacteria that was not attributable to decreased infection rates. With IL-2, the reduction was most often apparent only during the first 24 h after infection, while inhibition with IFN-gamma was apparent throughout the culture period of 48 h. The addition of either IL-2 or IFN-gamma to macrophage cultures also resulted in reduced intracellular CFU of the virulent B. abortus strain 2308 and the attenuated rough mutant B. abortus strain RB51. Inhibition of intracellular growth was not augmented by combinations of cytokines. Additional studies with IFN-gamma and IL-2 indicated that they could mediate the inhibition of intracellular growth of B. abortus in resident and thioglycolate broth-induced BALB/c peritoneal macrophages and in splenic macrophages. IFN-gamma also inhibited bacterial growth when added after infection of the macrophages, although the magnitude of the antibrucellae effects was less than that when it was added before infection. Furthermore, the maximal inhibitory effect was sustained only when IFN-gamma remained in the cultures after infection of the macrophages.