Preservation of cell cycle characteristics in solid tumor in vitro.
Preservation of cell cycle characteristics in solid tumor in vitro.
复制标题
体外实体瘤中细胞周期特征的保存。
作者：
K. Sasaki;M. Takahashi
本文采用体外短期培养法分析了肾癌HKC-400实体瘤的细胞周期参数，并与动物实验结果进行了比较。在高压氧下用[3 H]胸苷标记组织碎片。连续标记指数以与体内实验相同的速率线性增加，并且由该方法获得的S期长度与标记有丝分裂分数法获得的S期长度一致。从连续标记的有丝分裂曲线测量G2 + M期的持续时间，发现与体内数据一致。这些结果表明，细胞周期的进程没有被阻止在体外至少在有限的时间后解释，和可能的临床应用进行了讨论。
Cell cycle parameters of explanted solid tumor (renal carcinoma HKC-400) were analyzed by short-term incubation in vitro and compared with those measured in the animal. The tissue fragments were labeled with [3H]thymidine under hyperbaric oxygen. Continuous labeling index increased linearly at the same rate as in the in vivo experiment, and the S-phase length by this method coincided with that obtained by the fraction of labeled mitoses method. Duration of G2 + M phases was measured from the continuously labeled mitoses curve and was found to agree with the in vivo data. These results indicated that cell cycle progression was not blocked in vitro at least for a limited time after explanation, and possible clinical application of this technique ws discussed.