Position specific growth regulation of 3T3 cells in vivo.
Position specific growth regulation of 3T3 cells in vivo.
复制标题
体内 3T3 细胞的位置特异性生长调节。
DOI：
10.1016/0012-1606(92)90008-5
复制
发表时间：
1992
影响因子：
2.7
通讯作者：
Muneoka,K
中科院分区：
文献类型：
--
作者：
Trevino,C;Calof,A;Muneoka,K
在这项研究中，我们已经调查的机制，通过监测3 T3细胞，引入到发育中的小鼠肢体使用exouterosurgery的空间生长进行调节。用人细胞表面糖蛋白CD 8标记3 T3细胞，并注射到7-9期小鼠肢体中。在注射后24和48小时，用[3 H]胸苷标记胚胎，并进行免疫组织化学和放射自显影。将CD 8阳性细胞的标记指数与邻近肢芽细胞的标记指数以及注射部位在肢内的位置进行比较。我们发现，3 T3细胞的标记指数是在雅阁与肢体细胞，直接围绕他们，3 T3细胞显示高标记指数在肢体地区的高增长和低标记指数在肢体地区的低增长。此外，我们发现，肢芽细胞和注射的3 T3细胞显示一般近端（低）到远端（高）梯度的生长在分析的阶段。我们从这些结果中得出结论，位置特异性调节生长发生在一个非细胞自主的方式，很可能是由有丝分裂信号，是本地化的肢体环境。此外，我们的研究结果表明，利用已建立的细胞系作为体内探针监测发育机制的有用性。
In this study we have investigated the mechanism by which spatial growth is regulated by monitoring 3T3 cells, introduced into the developing mouse limb usingexo uterosurgery. The 3T3 cells were labeled with a human cell surface glycoprotein, CD8, and injected into stage 7–9 mouse limbs. At 24 and 48 hr after injection embryos were labeled with [3H]thymidine and processed for immunohistochemistry and autoradiography. The labeling index of CD8 positive cells was compared to that of neighboring limb bud cells and also to the position of the injection site within the limb. We find that the labeling index of 3T3 cells is in accord with that of the limb cells that immediately surround them; 3T3 cells display a high labeling index in limb regions of high growth and a low labeling index in limb regions of low growth. In addition, we find that both limb bud cells and injected 3T3 cells display a general proximal (low) to distal (high) gradient of growth at the stages analyzed. We conclude from these results that position-specific regulation of growth occurs in a non-cell autonomous manner and is likely to be mediated by mitogenic signals that are localized within the limb environment. In addition, our results demonstrate the usefulness of utilizing established cell lines asin vivoprobes to monitor developmental mechanisms.