Culture and Characterization of Mesenchymal Stem Cells From Human Gingival Tissue
Culture and Characterization of Mesenchymal Stem Cells From Human Gingival Tissue
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DOI：
10.1902/jop.2010.090566
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发表时间：
2010-06-01
影响因子：
4.3
通讯作者：
Sanz, Antonio
中科院分区：
文献类型：
--
作者：
Mitrano, Tomas I.;Grob, Melisa S.;Sanz, Antonio
背景：利用间充质干细胞（MSCs）进行组织工程是一种新的治疗方式，具有几个优点。MSC具有高增殖潜力，并且可以在移植前进行操作以允许分化，这表明它们可能是再生程序的理想候选者。精确鉴定能够再生牙周组织的细胞是有价值的，因为还没有描述可预测的再生过程。本研究的目的是确定人牙龈结缔组织中存在的间充质干细胞及其形态和功能的characteristics.Methods：牙龈结缔组织样本从5名健康学生。样本被深层上皮化，仅留下结缔组织。将外植体切碎并在组织培养皿上培养3至4周，之后通过流式细胞术表征细胞。通过培养物染色诱导和评价成骨、成软骨和成脂谱系的分化。免疫调节试验也performed.Results：结果表明，牙龈组织细胞符合国际细胞治疗学会提出的最低标准被定义为MSC。细胞表征对CD 90、CD 105、CD 73、CD 44和CD 13标志物始终呈阳性，对造血标志物CD 34、CD 38、CD 45和CD 54呈阴性。我们观察了成脂、成软骨和成骨谱系的阳性染色的分化。结论：牙龈结缔组织可以作为间充质干细胞的储存库，用于组织工程的再生过程。牙周病学杂志2010;81：917-925。
Background: Tissue engineering using mesenchymal stem cells (MSCs) is a recent therapeutic modality that has several advantages. MSCs have high proliferation potential and may be manipulated to permit differentiation before being transplanted, suggesting they may be an ideal candidate for regenerative procedures. Precise identification of cells capable of regenerating the periodontium is valuable because no predictable regeneration procedure has yet been described. The purpose of this study is to determine the presence of MSCs in human gingival connective tissue and their morphologic and functional characteristics.Methods: Gingival connective tissue samples were obtained from five healthy students. The samples were deepithelialized, leaving only connective tissue. The explants were minced and cultured on tissue culture dishes for 3 to 4 weeks, after which cells were characterized by flow cytometry. Differentiation into osteogenic, chondrogenic, and adipogenic lineages was induced and evaluated by culture staining. An immunoregulation assay was also performed.Results: The results show that gingival tissue cells fulfill the minimal criteria proposed by the International Society for Cellular Therapy to be defined as MSCs. Cell characterization was consistently positive for CD90, CD105, CD73, CD44, and CD13 markers and negative for hematopoietic markers CD34, CD38, CD45, and CD54. We observed differentiation in positive staining of adipogenic, chondrogenic, and osteogenic lineages. Furthermore, gingival cells showed immunomodulative capacity.Conclusion: Gingival connective tissue could be a reservoir of MSCs that could be used in regenerative procedures based on tissue engineering. J Periodontot 2010;81:917-925.