Differential antagonistic responses of Bacillus pumilus MSUA3 against Rhizoctonia solani and Fusarium oxysporum causing fungal diseases in Fagopyrum esculentum Moench
Differential antagonistic responses of Bacillus pumilus MSUA3 against Rhizoctonia solani and Fusarium oxysporum causing fungal diseases in Fagopyrum esculentum Moench
复制标题
DOI：
10.1016/j.micres.2017.08.012
复制
发表时间：
2017-01-01
影响因子：
6.7
通讯作者：
Bajpai, Vivek K.
中科院分区：
文献类型：
--
作者：
Agarwal, Mohit;Dheeman, Shrivardhan;Bajpai, Vivek K.
本通讯研究了几丁质酶和表面素介导的番茄根丝核菌和尖孢镰刀菌的生物防治。短小芽孢杆菌MSUA3作为一种潜在的细菌菌株，通过几丁质水解酶和一种抗生素表面素，对茄芽孢杆菌和氧化孢子菌的生长有很强的抑制作用。植物生长促进属性似乎涉及到植物生长促进和产量属性。无细胞培养上清（CFCS）即使在热处理（煮沸/蒸压）的CFCS中，也对尖孢镰刀菌和茄枯病菌有一定的杀灭作用。菌落PCR扩增结果显示，表面素可能参与疾病控制。几丁质水解酶和抗生素表面素证明了小分枝杆菌MSUA3对尖孢镰刀菌和番茄枯萎病菌的不同生物防治效果。利用蛭石为基础的生物制剂可显著降低苦荞麦的病害指数，提高产量，表明苦荞麦MSUA3是一种成功的潜在生物防治剂（BCA）和有效的植物促生根瘤菌（PGPR）。
Chitinase and surfactin-mediated biocontrol of Rhizoctonia solani and Fusarium oxysporwn causing wilt and root rot of Fagopyrwn esculentum respectively has been studied in this communication. Bacillus pumilus MSUA3 as a potential bacterial strain strongly inhibited the growth of R. solani and F. oxysporwn involving the chitinolytic enzymes and an antibiotic surfactin. Plant growth promoting attributes seem to be involved in plant growth promotion and yield attributes. The action of cell-free culture supernatant (CFCS) was found deleterious to F. oxysporum and R. solani even in the heat-treated (boiled/autoclaved) CFCS. The possible involvement of surfactin in disease control was revealed by colony PCR amplification of SrfA. Chitinolytic enzyme and antibiotic surfactin evidenced differential biocontrol of F. oxysporum and R. solani by B. pumilus MSUA3. A significant reduction in disease index under gnotobiotic conditions and productivity enhancement of F. esculentum using vermiculite-based bioformulation revealed B. pumilus MSUA3 as a successful potential biocontrol agent (BCA) and an efficient plant growth promoting rhizobacterium (PGPR) for disease management and productivity enhancement of buckwheat crop.