Exercise and T-lymphocyte function: a comparison of proliferation in PBMC and NK cell-depleted PBMC culture
Exercise and T-lymphocyte function: a comparison of proliferation in PBMC and NK cell-depleted PBMC culture
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DOI：
10.1152/japplphysiol.00926.2001
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发表时间：
2002-06-01
影响因子：
3.3
通讯作者：
Mackinnon, LT
中科院分区：
文献类型：
--
作者：
Green, KJ;Rowbottom, DG;Mackinnon, LT
本研究利用最近开发的微珠技术，从外周血单核细胞（PBMC）制剂中去除自然杀伤（NK）细胞，以确定急性运动对T淋巴细胞功能的影响，独立于淋巴细胞亚群的变化。12名训练有素的男性跑步者完成了60分钟的运动试验，在95%的训练阈值和无运动对照试验。在每个阶段采集6份血液样本：运动前，运动中期，运动后立即，运动后30，60和90分钟。用促分裂原植物血凝素刺激分离的PBMC和NK细胞耗尽的PBMC。通过使用3-（4，5-二甲基噻唑-2-基）-2，5-二苯基四唑溴化物染料摄取来评估细胞增殖。在PBMC培养物中，有一个显着较低的有丝分裂原植物血凝素的反应，在运动后立即与对照条件相比。在NK细胞耗尽的PBMC培养物或针对CD 3细胞百分比调整的应答中，对照和运动条件之间没有显著差异。目前的研究结果不支持运动后T淋巴细胞功能降低的观点。
This study utilized recently developed microbead technology to remove natural killer (NK) cells from peripheral blood mononuclear cell (PBMC) preparations to determine the effect of acute exercise on T-lymphocyte function, independent of changes in lymphocyte subpopulations. Twelve well-trained male runners completed a 60-min exercise trial at 95% ventilatory threshold and a no-exercise control trial. Six blood samples were taken at each session: before exercise, midexercise, immediately after exercise, and 30, 60, and 90 min after exercise. Isolated PBMC and NK cell-depleted PBMC were stimulated with the mitogen phytohemagglutinin. Cellular proliferation was assessed by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide dye uptake. In the PBMC cultures, there was a significantly lower mitogen response to phytohemagglutinin in exercise compared with the control condition immediately postexercise. There were no significant differences between the control and exercise conditions in NK cell-depleted PBMC cultures or in the responses adjusted for the percentage of CD3 cells. The present findings do not support the view that T-lymphocyte function is reduced after exercise.