Genetic and Experimental Evidence Supports the Continuum of the Central Extended Amygdala and a Mutiple Embryonic Origin of Its Principal Neurons
Genetic and Experimental Evidence Supports the Continuum of the Central Extended Amygdala and a Mutiple Embryonic Origin of Its Principal Neurons
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DOI：
10.1002/cne.22719
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发表时间：
2011-12-01
影响因子：
2.5
通讯作者：
Medina, Loreta
中科院分区：
文献类型：
--
作者：
Bupesh, Munisamy;Abellan, Antonio;Medina, Loreta
中央延伸的杏仁核是端脑控制摄食、恐惧反应、压力和焦虑的主要输出中心。尽管有丰富的数据支持沿着延伸的杏仁核在神经化学、连接和功能上的相似性，但对这一连续体的胚胎学支持是缺乏的。采用体外迁移分析、原位杂交和免疫组织化学染色相结合的方法，我们发现其主要成分，包括杏仁中央核和终纹外侧床核(BST)，是由不同比例的背侧外侧神经节隆起(LGE)、腹侧LGE和内侧神经节隆起(MGE)来源的主神经元组成的嵌合体。背侧LGE产生Pax6表达的神经元，主要分布在中央延伸杏仁核的外侧部分，包括包膜和中央杏仁外侧核的一部分，但也产生少数细胞用于外侧BST。根据与前脑啡肽原的相关性，这些细胞中的许多可能是脑啡肽能细胞。腹侧LGE产生表达Islet1的神经元，这些神经元主要分布在中央杏仁核的中央和内侧部分，但也为外侧BST产生大量神经元。与促肾上腺皮质激素释放因子的相关性表明，这些神经元表达这种神经肽。MGE产生外侧BST的大部分神经元，但其腹尾亚群也产生重要的投射神经元亚群，其中包含中央杏仁核内侧的生长抑素。因此，不同的主神经元起源于不同的胚胎域，但相同的域为中央延伸杏仁核的大多数细分贡献神经元，这可能解释了神经化学和走廊沿线联系的相似性。J.Comp.神经。519：3507-3531,2011。(C)2011年Wiley-Liss，Inc.
The central extended amygdala is the major output center for telencephalic control of ingestion, fear responses, stress, and anxiety. In spite of the abundant data supporting the similarity in neurochemistry, connections, and function along the extended amygdala, embryological support for this continuum is lacking. By using a combination of in vitro migration assays, in situ hybridization, and immunostaining, here we show that its major components, including central amygdala and lateral bed nucleus of the stria terminalis (BST), are mosaics formed by different proportions of dorsal lateral ganglionic eminence (LGE)-, ventral LGE-, and medial ganglionic eminence (MGE)-derived principal neurons. The dorsal LGE produces Pax6-expressing neurons that primarily populate lateral parts of the central extended amygdala, including the capsular and part of lateral central amygdala, but also produces a few cells for the lateral BST. Based on correlation with preproenkephalin, many of these cells are likely enkephalinergic. The ventral LGE produces Islet1-expressing neurons that populate primarily the central and medial parts of the central amygdala but also produces numerous neurons for the lateral BST. Correlation with corticotropin-releasing factor suggests that these neurons express this neuropeptide. The MGE produces the majority of neurons of the lateral BST, but its ventrocaudal subdivision also produces an important subpopulation of projection neurons containing somatostatin for medial aspects of the central amygdala. Thus, distinct principal neurons originate in different embryonic domains, but the same domains contribute neurons to most subdivisions of the central extended amygdala, which may explain the similarity in neurochemistry and connections along the corridor. J. Comp. Neurol. 519:3507-3531, 2011. (C) 2011 Wiley-Liss, Inc.