The effect of aspartate on the energy metabolism in the liver of weanling pigs challenged with lipopolysaccharide
The effect of aspartate on the energy metabolism in the liver of weanling pigs challenged with lipopolysaccharide
复制标题
天门冬氨酸对脂多糖攻击断奶仔猪肝脏能量代谢的影响
DOI：
10.1007/s00394-014-0739-3
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发表时间：
2015-06-01
影响因子：
5
通讯作者：
Yi, Dan
中科院分区：
文献类型：
--
作者：
Kang, Ping;Liu, Yulan;Yi, Dan
目的探讨天冬氨酸（Asp）对脂多糖（LPS）攻击仔猪肝脏能量状态的改善作用（对照饮食和盐水处理）;（2）LPS激发对照（相同的对照饮食和LPS激发）;（3）LPS +0.5%Asp处理（0.5%Asp饮食和LPS激发）;和（4）LPS +1.0%Asp处理（1.0%Asp饮食和LPS激发）。第19天，分别腹腔注射100 μg/kg体重的大肠杆菌内毒素和等体积的0.9%NaCl溶液。所有猪在LPS或生理盐水注射后24 h屠宰，并收集肝脏用于进一步analysis.ResultsDietary补充Asp改善肝脏能量状态证明的ATP浓度和腺苷酸能量电荷的增加，和AMP浓度和AMP/ATP比值的降低（p< 0.05）。补充Asp可增加肝脏糖酵解和三羧酸（TCA）循环中关键酶丙酮酸激酶和柠檬酸合成酶的mRNA表达（p< 0.05），并有增加肝脏丙酮酸脱氢酶和异柠檬酸脱氢酶β mRNA表达的趋势（p< 0.10）。此外，Asp还可增加肝脏AMPK α1、AMPKα2、沉默信息调节因子Sirt 1和增殖物激活受体γ辅激活因子1α（PGC 1 α）的mRNA表达（p< 0.05）。结论补充天冬氨酸可改善脂多糖损伤肝脏的能量状态，其机制可能与激活TCA循环和糖酵解代谢途径，激活AMPK信号通路有关。
AbstractsPurposeThis study was conducted to investigate whether aspartate (Asp) could improve liver energy status in the lipopolysaccharide (LPS)-challenged pigs.MethodsTwenty-four weaned pigs were assigned to four treatments: (1) nonchallenged control (control diet and saline-treated); (2) LPS-challenged control (the same control diet and LPS-challenged); (3) LPS + 0.5 % Asp treatment (0.5 % Asp diet and LPS-challenged); and (4) LPS + 1.0 % Asp treatment (a 1.0 % Asp diet and LPS-challenged). On d 19, the pigs were injected intraperitoneally withEscherichia coliLPS at 100 μg/kg body weight, and the same volume of 0.9 % NaCl solution, respectively. All pigs were slaughtered at 24 h after LPS or saline injection, and the liver was collected for further analysis.ResultsDietary supplementation with Asp improved liver energy status evidenced by the increased ATP concentration and adenylate energy charges, and the decreased AMP concentration and AMP/ATP ratio (p< 0.05). Asp supplementation increased the mRNA expression of key enzymes in hepatic glycolysis and tricarboxylic acid (TCA) cycle, including pyruvate kinase and citrate synthase (p< 0.05), and had a tendency to increase hepatic pyruvate dehydrogenase and isocitrate dehydrogenase β mRNA expression (p< 0.10). In addition, Asp increased the mRNA expressions of hepatic AMP-activated protein kinase (AMPK) α1, AMPKα2, silent information regulator (Sirt1), and proliferator-activated receptor-γ coactivator 1α (PGC1α) (p< 0.05). Moreover, Asp increased AMPKα phosphorylation (p< 0.05).ConclusionsThese results indicated that dietary supplementation of Asp could improve energy status in LPS-injured liver, which might result from motivating the metabolism pathway of TCA cycle and glycolysis and stimulating the AMPK signaling pathway.