Spatial control of calcineurin in response to heat shock in fission yeast.
Spatial control of calcineurin in response to heat shock in fission yeast.
复制标题
裂殖酵母中钙调神经磷酸酶响应热休克的空间控制。
DOI：
10.1111/gtc.12203
复制
发表时间：
2015
期刊：
影响因子：
2.1
通讯作者：
Reiko Sugiura
中科院分区：
文献类型：
--
作者：
Mari Higa;Ayako Kita;Kanako Hagihara;Yuki Kitai;Akira Doi;Rie Nagasoko;Ryosuke Sato;Reiko Sugiura
在裂殖酵母中，Ppb1（Ca2+/钙调蛋白依赖性蛋白磷酸酶钙调神经磷酸酶）调节多种生物过程，例如胞质分裂、Ca2+稳态、膜运输和细胞壁完整性。钙调神经磷酸酶使 Prz1 转录因子去磷酸化，导致其核转位和 CDRE（钙调神经磷酸酶依赖性反应元件）控制下的基因表达。尽管钙调磷酸酶介导的下游转录因子的空间控制已在许多生物体中得到深入研究，但人们对钙调磷酸酶对应激的空间调节知之甚少。在这里，我们发现热激刺激了 Prz1 的钙调磷酸酶依赖性核转位和 CDRE 依赖性基因表达。值得注意的是，钙调神经磷酸酶在亚细胞定位方面表现出巨大的变化，在热激时从弥散的细胞质结构转移到点状结构。钙调神经磷酸酶点分别与 P 体或应激颗粒的成分 Dcp2 或 Pabp 共定位，因此表明钙调神经磷酸酶是热激下 RNA 颗粒的组成部分。重要的是，钙调磷酸酶抑制剂 FK506 显着抑制钙调磷酸酶颗粒的积累，而组成型活性钙调磷酸酶在热休克时强烈积聚在颗粒中，这表明磷酸酶活性对于钙调磷酸酶定位很重要。值得注意的是，钙调神经磷酸酶的消耗导致 Nrd1 和 Pabp 阳性 RNA 颗粒的快速出现。将讨论钙调磷酸酶在热休克反应中的可能作用。
In fission yeast, Ppb1, the Ca2+/calmodulin‐dependent protein phosphatase calcineurin regulates multiple biological processes, such as cytokinesis, Ca2+‐homeostasis, membrane trafficking and cell wall integrity. Calcineurin dephosphorylates the Prz1 transcription factor, leading to its nuclear translocation and gene expression under the control of CDRE (calcineurin‐dependent response element). Although the calcineurin‐mediated spatial control of downstream transcription factors has been intensively studied in many organisms, less is known about the spatial regulation of calcineurin on stresses. Here, we show that heat shock stimulates calcineurin‐dependent nuclear translocation of Prz1 and CDRE‐dependent gene expression. Notably, calcineurin exhibited a dramatic change in subcellular localization, translocating from diffuse cytoplasmic to dot‐like structures on heat shock. The calcineurin dots colocalized with Dcp2 or Pabp, the constituent of P‐bodies or stress granules, respectively, thus suggesting that calcineurin is a component of RNA granules under heat shock. Importantly, the calcineurin inhibitor FK506 markedly inhibited the accumulation of calcineurin granules, whereas the constitutively active calcineurin strongly accumulated in the granules on heat shock, suggesting that phosphatase activity is important for calcineurin localization. Notably, the depletion of calcineurin induced a rapid appearance of Nrd1‐ and Pabp‐positive RNA granules. The possible roles of calcineurin in response to heat shock will be discussed.