Isolation and functional characterization of the FLOWERING LOCUS T homolog, the LsFT gene, in lettuce
Isolation and functional characterization of the FLOWERING LOCUS T homolog, the LsFT gene, in lettuce
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DOI：
10.1016/j.jplph.2011.02.004
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发表时间：
2011-09-01
影响因子：
4.3
通讯作者：
Honda, Ichiro
中科院分区：
文献类型：
--
作者：
Fukuda, Machiko;Matsuo, Satoshi;Honda, Ichiro
高温诱导生菜抽苔在农业上是不可取的，因此寻找控制生菜从营养生长向生殖生长转变的机制很重要。开花基因T(FT)在几种植物的开花诱导中起着重要作用。为了阐明生菜成花诱导，我们从生菜中分离到FT基因(LsFT)。序列分析和系统发育关系表明，LsFT基因与拟南芥、番茄等物种的FT基因具有较高的同源性。LsFT诱导转基因拟南芥提前开花，但与AtFT相比并不完全有效。在开花诱导条件下(较高温度)，LsFT基因在最大的叶片中含量丰富。基因表达与顶端分生组织的花分化相关。我们的结果表明，LsFT在生菜中可能是一个FT同源物，与拟南芥中的同源物相似，它调节花的转换。这是关于生菜花基因的第一个信息，用于阐明生菜开花转换的调控。(C)2011年爱思唯尔股份有限公司。版权所有。
High temperature-induced bolting of lettuce is undesirable agriculturally, making it important to find the mechanism governing the transition from vegetative to reproductive growth. FLOWERING LOCUS T (FT) genes play important roles in the induction of flowering in several plant species. To clarify floral induction in lettuce, we isolated the FT gene (LsFT) from lettuce. Sequence analysis and phylogenetic relationships of LsFT revealed considerable homology to FT genes of Arabidopsis, tomato, and other species. LsFT induced early flowering in transgenic Arabidopsis, but was not completely effective compared to AtFT. LsFT mRNA was abundant in the largest leaves under flowering-inducible conditions (higher temperatures). Gene expression was correlated with flower differentiation of the shoot apical meristem. Our results suggest that LsFT is a putative FT homolog in lettuce that regulates flower transition, similar to its homolog in Arabidopsis. This is the first information on the lettuce floral gene for elucidating regulation of the flowering transition in lettuce. (C) 2011 Elsevier GmbH. All rights reserved.