Generative cell-specific activation of the histone gH2A gene promoter of Lilium longiflorum in tobacco.
Generative cell-specific activation of the histone gH2A gene promoter of Lilium longiflorum in tobacco.
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烟草中长花百合组蛋白 gH2A 基因启动子的生成细胞特异性激活。
DOI：
10.1007/s00497-012-0194-3
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发表时间：
2012
影响因子：
--
通讯作者：
Inoue M
中科院分区：
文献类型：
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作者：
Ueda K.;Ono M.;Iwashita J.;Wabiko H.;Inoue M
长花百合 gH2A 启动子仅在表达 gH2A 启动子::GUS（β-葡萄糖醛酸酶）构建体作为报告基因的转基因烟草的成熟花粉的生殖细胞中具有活性。在转基因烟草花粉发育期间检查的gH2A启动子活性的时间和空间方面表明，直到发育花粉进入早期双细胞发育阶段才检测到GUS报告基因活性。活性首先在早中期的生殖细胞中检测到，并在双细胞中期逐渐增加到最高水平。烟草中GUS活性的出现和寿命模式与百合花粉发育过程中gH2AmRNA的模式非常相似。使用秋水仙碱（一种众所周知的微管解聚剂）进行外源治疗，可阻断小孢子有丝分裂并抑制生殖细胞分化。在所得的异常花粉中未检测到 GUS 信号，该花粉缺乏生殖细胞分化。这些数据强烈表明正常的生殖细胞发育对于gH2A启动子的激活是必需的。此外，这些结果表明gH2A启动子的共同转录激活因子可能存在于百合和烟草中，并且此类推定因子仅在生殖细胞经历正常发育时激活gH2A启动子。
TheLilium longiflorum gH2Apromoter is active exclusively in the generative cells of mature pollen in transgenic tobacco expressing thegH2Apromoter::GUS(β-glucuronidase) construct as a reporter gene. Temporal and spatial aspects ofgH2Apromoter activity examined during pollen development in transgenic tobacco reveal that GUS reporter activity was not detected until developing pollen entered the early bicellular developmental stage. Activity was first detected in generative cells at early-mid stages and gradually increased to maximum levels at mid-bicellular stages. The patterns of appearance and longevity of GUS activity in tobacco were very similar to those ofgH2AmRNA during pollen development inLilium. Exogenous treatment with colchicine, a well-known microtubule depolymerize, blocked microspore mitosis and inhibited generative cell differentiation. No GUS signal was detected in the resulting anomalous pollen, which lacked generative cell differentiation. These data strongly suggest that normal generative cell development is essential for activation of thegH2Apromoter. Furthermore, these results indicate that common transcriptional activator(s) of thegH2Apromoter may be present in bothLiliumandNicotiana, and that such putative factor(s) activates thegH2Apromoter only when generative cells undergo normal development.