Expression of the chloroplast-localized small heat shock protein by oxidative stress in rice
Expression of the chloroplast-localized small heat shock protein by oxidative stress in rice
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DOI：
10.1016/s0378-1119(00)00043-3
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发表时间：
2000-03-21
期刊：
影响因子：
3.5
通讯作者：
Jo, J
中科院分区：
文献类型：
--
作者：
Lee, BH;Won, SH;Jo, J
一种水稻（Oryza sativa L.）CV. Nakdong）的cDNA克隆Oshsp 26，其编码叶绿体定位的小热休克蛋白（smHSP）。基因组DNA的Southern杂交分析和cDNA文库的筛选结果表明，Oshsp 26基因在水稻基因组中由一个单基因编码。Oshsp26基因在高温胁迫下表达：42 ℃高温处理2 h时表达量最高，20 min后可检测到，2 h后表达量达到最高。还发现Oshsp26基因在氧化应激后表达，即使在没有热应激的情况下。甲基紫精（MV）在光和过氧化氢（H2O2）处理水稻植株，无论是在光或在黑暗中，都引起了显着的积累的成绩单和蛋白质。由于在光照下MV处理导致叶绿体内H2O2的产生，因此H2O2本身可能起诱导Oshsp26基因表达的作用。这些结果表明，叶绿体smHSP在保护叶绿体免受氧化胁迫以及热胁迫造成的损害中起着重要的作用。(C)2000 Elsevier Science B.V.保留所有权利。
A rice (Oryza sativa L. cv. Nakdong) cDNA clone, Oshsp26, encoding the chloroplast-localized small heat shock protein (smHSP) was isolated. Southern blot analysis of genomic DNA and the result of screening of a cDNA library indicated that the Oshsp26 gene is encoded by a single gene in the rice genome. The Oshsp26 gene was expressed following heat stress: the transcript level was highest when rice leaves were treated at high temperatures for 2 h at 42 degrees C, and the transcripts became detectable after 20 min and reached a maximum level after 2 h. It was also found that the Oshsp26 gene was expressed following oxidative stress even in the absence of heat stress. Treatment of rice plants with methyl viologen (MV) in the light and treatment with hydrogen peroxide (H2O2), either in the light or in the dark, both caused a significant accumulation of the transcripts and the protein. Since MV treatment in the light leads to the generation of H2O2 inside the chloroplast, it, is likely that H2O2 by itself acts to induce the expression of the Oshsp26 gene. These results suggest that the chloroplast smHSP plays an important role in protecting the chloroplast against damage caused by oxidative stress as well as by heat stress. (C) 2000 Elsevier Science B.V. All rights reserved.