Coexpression of Notch3 and Rgs5 in the pericyte-vascular smooth muscle cell axis in response to pulp injury
Coexpression of Notch3 and Rgs5 in the pericyte-vascular smooth muscle cell axis in response to pulp injury
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DOI：
10.1387/ijdb.072393hl
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发表时间：
2007-01-01
影响因子：
0.7
通讯作者：
Kjeldsen, Annette L.
中科院分区：
文献类型：
--
作者：
Lovschall, Henrik;Mitsiadis, Thimios A.;Kjeldsen, Annette L.
最近的研究表明，人类牙齿的牙髓中含有一群具有干细胞特性的细胞，有人认为这些细胞起源于周细胞。Notch信号通路的分子调节干细胞的命运，而RGS5是周细胞的一个很好的标记。牙科创伤、龋损等病理条件刺激牙髓干细胞形成修复性牙本质。先前的研究表明，参与Notch途径的基因在啮齿动物和人类的牙髓损伤中被激活。为了证明周细胞在牙齿修复中作为干细胞来源的重要性，我们用原位杂交的方法研究了RGS5和Notch3mRNA在发育中的、成年完整的和受损的啮齿动物牙齿中的表达。此外，我们还用免疫组织化学方法检测了Notch3蛋白在龋损和牙齿损伤中的分布。RGs5和Notch3在啮齿动物牙齿发育过程中以及损伤后即刻呈现重叠表达模式。这两个基因在发育过程中的血管结构以及受伤牙齿的血管周围和单个毛细血管细胞中都有表达。然而，RGs5和Notch3在牙齿修复过程中的表达模式不同，RGs5在牙髓中央小动脉沿细胞-血管平滑肌细胞轴线的表达相对广泛。这些结果表明RGS5和Notch3在发育中和损伤的牙齿周细胞中共同表达，并进一步表明血管来源的干细胞在牙髓愈合中的重要性。
Recent studies have shown that the pulp of human teeth contains a population of cells with stem cell properties and it has been suggested that these cells originate from pericytes. Molecules of the Notch signaling pathway regulate stem cell fate specification, while Rgs5 represents an excellent marker for pericytes. Pathological conditions such as dental trauma and carious lesion stimulate pulp stem cells to elaborate reparative dentin. Previous studies have shown that genes involved in the Notch pathway are activated in response to pulp injury in rodent and humans. To demonstrate the importance of pericytes as a source of stem cells during dental repair, we have studied Rgs5 and Notch3mRNA expression by in situ hybridization in developing, adult intact and injured rodent teeth. Furthermore, we have examined the distribution of Notch3 protein in carious and injured human teeth using immunohistochernistry. Overlapping expression patterns of Rgs5 and Notch3 were observed during rodent tooth development as well as immediately after injury. Both genes were expressed in vascular structures during development and in perivascular and single capillary cells of injured teeth. However, the expression patterns of Rgs5 and Notch3 were different during tooth repair, with relatively extensive Rgs5 expression along the pericyte-vascular smooth muscle cell axis in central pulp arterioles. These results show co-expression of Rgs5 and Notch3 in pericytes of developing and injured teeth and furthermore indicate the importance of vascular-derived stem cells during pulp healing.