Pancreatic Microcirculation Profiles in the Progression of Hypertension in Spontaneously Hypertensive Rats.
Pancreatic Microcirculation Profiles in the Progression of Hypertension in Spontaneously Hypertensive Rats.
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自发性高血压大鼠高血压进展中的胰腺微循环特征
DOI：
10.1093/ajh/hpaa164
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发表时间：
2021-02-18
影响因子：
3.2
通讯作者：
Xiu R
中科院分区：
文献类型：
--
作者：
Liu M;Song X;Wang B;Li Y;Li A;Zhang J;Zhang H;Xiu R
背景新出现的证据表明胰腺是降解蛋白酶活性的主要来源，并且在高血压条件下发生不受控制的蛋白水解受体裂解，这导致全身功能障碍和终末器官损伤。然而，在高血压的进展过程中胰腺微循环的变化仍然未知。方法测定5、8、13、18周龄自发性高血压大鼠（SHR）和正常血压对照Wistar京都大鼠胰腺微循环血流分布和微血管运动。采用小波变换将胰腺微血流动力学信号转换到时频域，构建三维谱尺度图。比较各组之间的特征振荡器，包括内皮，神经源性，肌源性，呼吸和心脏振荡器的振幅。使用Griess反应测量血浆亚硝酸盐/硝酸盐水平。此外，内皮素-1，丙二醛，超氧化物歧化酶，白细胞介素-6水平测定酶联免疫吸附试验。结果SHR表现出减少的血流分布模式，平均血流灌注、微血管运动幅度和频率进行性降低。小波变换频谱分析显示，从8至18周龄的SHR的内皮振荡器的振幅显着降低。此外，血液微循环化学补充部分解释了微血流动力学概况，如血浆亚硝酸盐/硝酸盐，内皮素-1，丙二醛和白细胞介素-6水平的增加和降低超氧化物歧化酶水平的SHR。结论：在SHR高血压进展过程中，胰腺微循环异常，包括血液分布模式紊乱，微血管运动受损，内皮振荡器振幅降低。
Abstract BACKGROUND Emerging evidence indicates that the pancreas serves as a major source of degrading protease activities and that uncontrolled proteolytic receptor cleavage occurs under hypertensive conditions, which leading to systemic dysfunction and end-organic damage. However, changes in pancreatic microcirculation profiles during the progression of hypertension remain unknown. METHODS Pancreatic microcirculatory blood distribution patterns and microvascular vasomotion of spontaneously hypertensive rats (SHRs) and normotensive control Wistar Kyoto rats at 5, 8, 13, and 18 weeks of age were determined. Wavelet transform analysis was performed to convert pancreatic microhemodynamic signals into time–frequency domains and construct 3-dimensional spectral scalograms. The amplitudes of characteristic oscillators including endothelial, neurogenic, myogenic, respiratory, and cardiac oscillators were compared among groups. Plasma nitrite/nitrate levels were measured using a Griess reaction. Additionally, endothelin-1, malondialdehyde, superoxide dismutase, and interleukin-6 levels were determined by enzyme-linked immunosorbent assay. RESULTS SHRs exhibited a reduced blood distribution pattern with progressively decreased average blood perfusion, amplitude, and frequency of microvascular vasomotion. Wavelet transform spectral analysis revealed significantly reduced amplitudes of endothelial oscillators from 8- to 18-week-old SHRs. Additionally, the blood microcirculatory chemistry complements explained the microhemodynamic profiles partially, as demonstrated by an increase in plasma nitrite/nitrate, endothelin-1, malondialdehyde, and interleukin-6 levels and a decreased superoxide dismutase level in SHRs. CONCLUSIONS Pancreatic microcirculation profiles are abnormal in the progression of hypertension in SHRs, including a disarranged blood distribution pattern, impaired microvascular vasomotion, and reduced amplitudes of endothelial oscillators.
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影响因子：
4.8
作者：
Kuzkaya, N;Weissmann, N;Dikalov, S
通讯作者：
Dikalov, S
影响因子：
6
作者：
Rosário, HS;Waldo, SW;Schmid-Schönbein, GW
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影响因子：
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作者：
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DOI：
10.1016/j.ijbiomac.2014.09.017
发表时间：
2015-01-01
影响因子：
8.2
作者：
Aragao, Danielle S.;de Andrade, Maria Claudina C.;Casarini, Dulce Elena
通讯作者：
Casarini, Dulce Elena
影响因子：
5.4
作者：
Junqueira, Camillo L. C.;Magalhaes, Maria Eliane C.;Bouskela, Eliete
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