Untranslated Regions of a Mobile Transcript Mediate RNA Metabolism
Untranslated Regions of a Mobile Transcript Mediate RNA Metabolism
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DOI：
10.1104/pp.109.144428
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发表时间：
2009-12-01
期刊：
影响因子：
7.4
通讯作者：
Hannapel, David J.
中科院分区：
文献类型：
--
作者：
Banerjee, Anjan K.;Lin, Tian;Hannapel, David J.
BEL1类转录因子广泛存在于植物中，与KNOTTED1相互作用，调节多种发育过程。马铃薯(Solanum tuberosum subsp.)Andigena)，BEL1类转录因子StBEL5及其Knox蛋白伙伴通过靶向控制生长的基因来调控块茎的形成。RNA检测方法和异种嫁接实验表明，StBEL5转录本存在于韧皮部细胞中，并跨越嫁接连接定位于块茎诱导的地上部。RNA的这种运动起源于叶脉和叶柄，受短日光周期诱导，受非翻译区调控，并与块茎产量的提高相关。对RNA迁移率的分析表明，5‘和3’非翻译区都有助于StBEL5 RNA的优先积累，但3‘非翻译区可能对从叶片到茎和茎中的运输有更大的贡献。将StBEL5非翻译区添加到另一个类似BEL1的mRNAs中，使其优先转运到茎尖，提高了块茎产量。转录稳定性分析表明，非翻译区和长日光周期提高了StBEL5 RNA在茎尖中的稳定性。当StBEL5的非翻译区与β-葡萄糖醛酸酶标记融合后，烟草原生质体中的翻译被含有3‘非翻译序列的构建体抑制。这些结果表明，StBEL5mRNA的非翻译区参与了其长距离运输、维持转录稳定性和控制翻译。
BEL1-like transcription factors are ubiquitous in plants and interact with KNOTTED1 types to regulate numerous developmental processes. In potato (Solanum tuberosum subsp. andigena), the BEL1-like transcription factor StBEL5 and its Knox protein partner regulate tuber formation by targeting genes that control growth. RNA detection methods and heterografting experiments demonstrated that StBEL5 transcripts are present in phloem cells and move across a graft union to localize in stolon tips, the site of tuber induction. This movement of RNA originates in leaf veins and petioles and is induced by a short-day photoperiod, regulated by the untranslated regions, and correlated with enhanced tuber production. Assays for RNA mobility suggest that both 5' and 3' untranslated regions contribute to the preferential accumulation of the StBEL5 RNA but that the 3' untranslated region may contribute more to transport from the leaf to the stem and into the stolons. Addition of the StBEL5 untranslated regions to another BEL1-like mRNA resulted in its preferential transport to stolon tips and enhanced tuber production. Transcript stability assays showed that the untranslated regions and a long-day photoperiod enhanced StBEL5 RNA stability in shoot tips. Upon fusion of the untranslated regions of StBEL5 to a beta-glucuronidase marker, translation in tobacco (Nicotiana tabacum) protoplasts was repressed by those constructs containing the 3' untranslated sequence. These results demonstrate that the untranslated regions of the mRNA of StBEL5 are involved in mediating its long-distance transport, in maintaining transcript stability, and in controlling translation.