Tumor necrosis factor-α (TNFα) inhibits progesterone and estradiol-17β production from cultured granulosa cells:: Presence of TNFα receptors in bovine granulosa and theca cells
Tumor necrosis factor-α (TNFα) inhibits progesterone and estradiol-17β production from cultured granulosa cells:: Presence of TNFα receptors in bovine granulosa and theca cells
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DOI：
10.1262/jrd.49.441
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发表时间：
2003-12-01
影响因子：
1.8
通讯作者：
Okuda, K
中科院分区：
文献类型：
--
作者：
Sakumoto, R;Shibaya, M;Okuda, K
本研究的目的是调查功能性肿瘤坏死因子-α（TNF α）受体是否存在于颗粒细胞和卵泡内膜细胞（卵泡膜细胞），从牛卵泡分为三组之一。每组定义为小泡状卵泡（小卵泡;直径3-5 mm），排卵前成熟卵泡（排卵前卵泡）或闭锁卵泡根据同侧或对侧卵巢和子宫中黄体的大体检查，（大小、颜色、稠度和粘液），以及卵泡液中孕酮（P-4）和雌二醇-17 β（E-2）浓度的比率。Scatchard分析显示，所有检查卵泡的颗粒细胞和卵泡膜细胞上均存在高亲和力结合位点（解离常数：4.7 +/- 0.15至6.9 +/- 1.40 nM）。闭锁卵泡颗粒细胞和卵泡膜细胞TNF α受体浓度显著高于排卵前卵泡细胞（P
The aim of this study was to investigate whether functional tumor necrosis factor-alpha (TNFalpha) receptors are present in the granulosa cells and the cells of theca interna (theca cells), obtained from bovine follicles classified into one of three groups. Each group was defined as either small vesicular ovarian follicles (small follicles; 3-5 mm in diameter), preovulatory mature ovarian follicles (preovulatory follicles) or atretic follicles (12-18 mm) according to gross examination of the corpus luteum in the epsilateral or contralateral ovary and the uterus (size, color, consistency and mucus), and the ratio of progesterone (P-4) and estradiol-17beta (E-2) concentrations in follicular fluid. A Scatchard analysis showed the presence of a high-affinity binding site on both granulosa and theca cells from all follicles examined (dissociation constant: 4.7 +/- 0.15 to 6.9 +/- 1.40 nM). Moreover, TNFalpha receptor concentrations in granulosa and theca cells obtained from atretic follicles were significantly higher than those in the cells from preovulatory follicles (P