Evidence for the linkage of the IGC H locus to a gene controlling the idiotypic specificity of anti-p-azophenylarsonate antibodies in strain A mice.
Evidence for the linkage of the IGC H locus to a gene controlling the idiotypic specificity of anti-p-azophenylarsonate antibodies in strain A mice.
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DOI：
10.1084/jem.137.1.22
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发表时间：
1973-01-01
影响因子：
15.3
通讯作者：
Potter, M
中科院分区：
文献类型：
--
作者：
Pawlak, L L;Mushinski, E B;Nisonoff, A;Potter, M
在所有测试的 A/J 品系小鼠中引发的抗对偶氮苯胂酸（抗 Ar）抗体具有一种或多种独特型特异性。在密切相关品系 AL/N 小鼠的抗 Ar 抗体中也发现了这些特异性，但在 BALB/c 小鼠的抗 Ar 抗体中没有发现。在同系小鼠中引发抗Ar抗体，其中控制重链恒定区同种异型标记的AL/N小鼠的IgCH基因座已在BALB/c背景上渐渗回交九代；然后使小鼠成为 AL/N 同种异型决定簇的纯合子。平均而言，这些抗体在交叉反应独特型含量方面与 AL/N 小鼠的抗体在数量上相当。这表明控制独特型的基因与IgCH基因座密切相关。由于独特型必须是 V 区序列的函数，因此结果表明 VH 和 CH 基因紧密连锁。几乎所有测试的 F1 小鼠（C57/BL x A/J 或 BALB/c x A/J）中都发现了交叉反应独特型。
Anti-p-azophenylarsonate (anti-Ar) antibodies elicited in all strain A/J mice tested share one or more idiotypic specificities. These specificities are also found in the anti-Ar antibodies of mice of the closely related strain, AL/N, but not in those of BALB/c mice. Anti-Ar antibodies were elicited in congenic mice in which the IgCH locus of AL/N mice, which controls allotypic markers in the constant regions of heavy chains, had been introgressively backcrossed for nine generations onto a BALB/c background; the mice were then rendered homozygous for the AL/N allotypic determinant. On the average, these antibodies were quantitatively equivalent, with respect to content of the cross-reactive idiotype, to those of AL/N mice. This indicates that the gene controlling the idiotype is closely linked to the IgCH locus. Since idiotype must be a function of V region sequences, the results suggest close linkage of VH and CH genes. The cross-reactive idiotype was found in nearly all F1 mice (C57/BL x A/J or BALB/c x A/J) tested.